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Fiber-FISH EST mapping is illustrated here. The red signal indicates hybridization of EST probes; the green signal comes from the underlying large insert clones. (Top) The centromeric side of the transcript map including the PWP2 and KNP1 genes. The center map includes the AIRE and C21orf3 genes. (Bottom) The telomeric part of the map including the SMT3A and C21orf1 genes. (For details, see Horelli-Kuitunen et al., p. 60.)