Published online before print
November 12, 2001
Genome Research, DOI: 10.1101/gr.209601
A Gene Expression Screen in Zebrafish Embryogenesis
Tetsuhiro
Kudoh,1
Michael
Tsang,1
Neil A.
Hukriede,
Xiongfong
Chen,2
Michael
Dedekian,
Christopher
J.
Clarke,
Anne
Kiang,
Stephanie
Schultz,
Jonathan A.
Epstein,2
Reiko
Toyama, and
Igor B.
Dawid3
Laboratory of Molecular Genetics and 2 Unit of Biological
Computation, National Institute of Child Health and Human Development,
National Institutes of Health, Bethesda, Maryland 20892, USA
A screen for developmentally regulated genes was conducted in the
zebrafish, a system offering substantial advantages for the study of
the molecular genetics of vertebrate embryogenesis. Clones from a
normalized cDNA library from early somitogenesis stages were picked
randomly and tested by high-throughput in situ hybridization for
restricted expression in at least one of four stages of development.
Among 2765 clones that were screened, a total of 347 genes with
patterns judged to be restricted were selected. These clones were
subjected to partial sequence analysis, allowing recognition of
functional motifs in 163 among them. In addition, a portion of the
clones were mapped with the aid of the LN54 radiation hybrid panel. The
usefulness of the in situ hybridization screening approach is
illustrated by describing several new markers for the characteristic
structure in the fish embryo named the yolk syncytial layer, and for
different regions of the developing brain.
1
These authors contributed equally to this work.
3
Corresponding author.
11:000-000 © by Cold Spring Harbor Laboratory Press ISSN 1088-9051/01 $5.00
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