Genome Research Econo tag

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Korenberg, J. R.
Right arrow Articles by Birren, B. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Korenberg, J. R.
Right arrow Articles by Birren, B. W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 9, Issue 5, 514-523, May 1999

RESOURCE
Mouse Molecular Cytogenetic Resource: 157 BACs Link the Chromosomal and Genetic Maps

Julie R. Korenberg,1,4 Xiao-Ning Chen,1 Keri L. Devon,2 David Noya,1 Mary L. Oster-Granite,3 and Bruce W. Birren2

1 Medical Genetics Birth Defects Center, Cedars-Sinai Medical Center, University of California, Los Angeles, Los Angeles, California 90048 USA; 2 Whitehead Institute/Massachusetts Institute of Technology (MIT) Center for Genome Research, Cambridge Massachusetts 02141 USA; 3 Department of Biomedical Science, University of California, Riverside, Riverside, California 92521 USA.

We have established a collection of strong molecular cytogenetic markers that span the mouse autosomes and X chromosome at an average spacing of one per 19 Mb and identify 127 distinct band landmarks. In addition, this Mouse Molecular Cytogenetic Resource relates the ends of the genetic maps to their chromosomal locations. The resource consists of 157 bacterial artificial chromosome (BAC) clones, each of which identifies specific mouse chromosome bands or band borders, and 42 of which are linked to genetic markers that define the centromeric and telomeric ends of the Whitehead/MIT recombinational maps. In addition, 108 randomly selected and 6 STS-linked BACs have been assigned to single chromosome bands. We have also developed a high-resolution fluorescent reverse-banding technique for mouse chromosomes that allows simultaneous localization of probes by fluorescence in situ hybridization (FISH) with respect to the cytogenetic landmarks. This approach integrates studies of the entire mouse genome. Moreover, these reagents will simplify gene mapping and analyses of genomic fragments in fetal and adult mouse models. As shown with the MMU16 telomeric marker for the trisomy 16 mouse model of Down syndrome, these clones can obviate the need for metaphase analyses. The potential contribution of this resource and associated methods extends well beyond mapping and includes clues to understanding mouse chromosomes and their rearrangements in cancers and evolution. Finally it will facilitate the development of an integrated view of the mouse genome by providing anchor points from the genetic to the cytogenetic and functional maps of the mouse as we attempt to understand mutations, their biological consequences, and gene function.

4   Corresponding author.

9:514-523 ©1999 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/99 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GeneticsHome page
K. E. Koehler, E. A. Millie, J. P. Cherry, S. E. Schrump, and T. J. Hassold
Meiotic Exchange and Segregation in Female Mice Heterozygous for Paracentric Inversions
Genetics, March 1, 2004; 166(3): 1199 - 1214.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
D Monk, L Bentley, C Beechey, M Hitchins, J Peters, M A Preece, P Stanier, and G E Moore
Characterisation of the growth regulating gene IMP3, a candidate for Silver-Russell syndrome
J. Med. Genet., August 1, 2002; 39(8): 575 - 581.
[Full Text] [PDF]

Home page
 Genome Res.Home page
L. C. Schalkwyk, B. Cusack, I. Dunkel, M. Hopp, M. Kramer, S. Palczewski, J. Piefke, S. Scheel, M. Weiher, G. Wenske, et al.
Advanced Integrated Mouse YAC Map Including BAC Framework
Genome Res., December 1, 2001; 11(12): 2142 - 2150.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
C. Dai, J. C. Celestino, Y. Okada, D. N. Louis, G. N. Fuller, and E. C. Holland
PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo
Genes & Dev., August 1, 2001; 15(15): 1913 - 1925.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Zhao
A comprehensive BAC resource
Nucleic Acids Res., January 1, 2001; 29(1): 141 - 143.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
S. Engemann, M. Strodicke, M. Paulsen, O. Franck, R. Reinhardt, N. Lane, W. Reik, and J. Walter
Sequence and functional comparison in the Beckwith-Wiedemann region: implications for a novel imprinting centre and extended imprinting
Hum. Mol. Genet., November 1, 2000; 9(18): 2691 - 2706.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
K. Osoegawa, M. Tateno, P. Y. Woon, E. Frengen, A. G. Mammoser, J. J. Catanese, Y. Hayashizaki, and P. J. de Jong
Bacterial Artificial Chromosome Libraries for Mouse Sequencing and Functional Analysis
Genome Res., January 1, 2000; 10(1): 116 - 128.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
M. Balbin, A. Fueyo, V. Knauper, J. M. Lopez, J. Alvarez, L. M. Sanchez, V. Quesada, J. Bordallo, G. Murphy, and C. Lopez-Otin
Identification and Enzymatic Characterization of Two Diverging Murine Counterparts of Human Interstitial Collagenase (MMP-1) Expressed at Sites of Embryo Implantation
J. Biol. Chem., March 23, 2001; 276(13): 10253 - 10262.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genes Dev. Learn. Mem.
Protein Science RNA Genome Res.