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Vol. 9, Issue 5, 457-462, May 1999

LETTER
An Automated Sample Preparation System for Large-Scale DNA Sequencing

Andre Marziali,1 Thomas D. Willis, Nancy A. Federspiel, and Ronald W. Davis

Stanford DNA Sequencing and Technology Center, Palo Alto, California 94304 USA

Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.

1   Corresponding author. Present address: Department of Physics and Astronomy, University of British Columbia, Vancouver, British Columbia V6T 1Z1 Canada.

9:457-462 ©1999 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/99 $5.00
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