Genome Research cityscape

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Research Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thacker, C.
Right arrow Articles by Rose, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thacker, C.
Right arrow Articles by Rose, A. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 9, Issue 4, 348-359, April 1999

LETTER
Functional Genomics in Caenorhabditis elegans: An Approach Involving Comparisons of Sequences from Related Nematodes

Colin Thacker,1 Marco A. Marra,2 Alana Jones,1 David L. Baillie,3 and Ann M. Rose1,4

1 Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, V6T 1Z3, Canada; 2 Genome Sequencing Center, Washington University, St. Louis, Missouri 63108 USA; 3 Institute of Molecular Biology and Biochemistry, Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, V5A 1S6, Canada

Comparative genomic analysis was used to investigate the gene structure of the bli-4 locus from two related Caenorhabditis species, C. elegans and C. briggsae. In C. elegans, bli-4 is a complex gene encoding a member of the kex2/subtilisin-like family of proprotein convertases. Genomic sequence comparisons coupled with RT-PCR analysis identified five additional coding exons that had not been identified previously using standard recombinant DNA techniques. The C. briggsae gene was able to rescue both viable blistered and developmentally arrested mutants of C. elegans bli-4, demonstrating functional conservation. In addition, deletion analysis of conserved sequences outside of coding regions, combined with phenotypic rescue experiments, identified regulatory elements that alter the expression of the bli-4 gene. These results demonstrate the utility of genomic sequence comparisons of homologous genes in related species as an effective tool with which to dissect the functional information of complex genes.

[The sequence for cosmid K0410 is available at GenBank (accession no. AFO 39719); fosmids G06P23 and G25K01 are available as online supplementary material at www.genome.org.]

4   Corresponding author.

9:348-359 ©1999 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/99 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 DevelopmentHome page
A.-F. Ruaud and J.-L. Bessereau
The P-type ATPase CATP-1 is a novel regulator of C. elegans developmental timing that acts independently of its predicted pump function
Development, March 1, 2007; 134(5): 867 - 879.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. D. Buchanan, P. E. Klein, and J. E. Mullet
Phylogenetic Analysis of 5'-Noncoding Regions From the ABA-Responsive rab16/17 Gene Family of Sorghum, Maize and Rice Provides Insight Into the Composition, Organization and Function of cis-Regulatory Modules
Genetics, November 1, 2004; 168(3): 1639 - 1654.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
N. Nikolaidis and M. Nei
Concerted and Nonconcerted Evolution of the Hsp70 Gene Superfamily in Two Sibling Species of Nematodes
Mol. Biol. Evol., March 1, 2004; 21(3): 498 - 505.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. G. Ayre, J. E. Blair, and R. Turgeon
Functional and Phylogenetic Analyses of a Conserved Regulatory Program in the Phloem of Minor Veins
Plant Physiology, November 1, 2003; 133(3): 1229 - 1239.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. B. Poole, J. Jin, and L. A. McReynolds
Cloning and Biochemical Characterization of Blisterase, a Subtilisin-like Convertase from the Filarial Parasite, Onchocerca volvulus
J. Biol. Chem., September 19, 2003; 278(38): 36183 - 36190.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Guo and S. P. Moose
Conserved Noncoding Sequences among Cultivated Cereal Genomes Identify Candidate Regulatory Sequence Elements and Patterns of Promoter Evolution
PLANT CELL, May 1, 2003; 15(5): 1143 - 1158.
[Abstract] [Full Text]

Home page
 Nucleic Acids ResHome page
T. Farrer, A. B. Roller, W. J. Kent, and A. M. Zahler
Analysis of the role of Caenorhabditis elegans GC-AG introns in regulated splicing
Nucleic Acids Res., August 1, 2002; 30(15): 3360 - 3367.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
A. Coghlan and K. H. Wolfe
Fourfold Faster Rate of Genome Rearrangement in Nematodes Than in Drosophila
Genome Res., June 1, 2002; 12(6): 857 - 867.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
D. L. Baillie and A. M. Rose
WABA Success: A Tool for Sequence Comparison between Large Genomes
Genome Res., August 1, 2000; 10(8): 1071 - 1073.
[Full Text]

Home page
 Genome Res.Home page
W. J. Kent and A. M. Zahler
Conservation, Regulation, Synteny, and Introns in a Large-scale C. briggsae-C. elegans Genomic Alignment
Genome Res., August 1, 2000; 10(8): 1115 - 1125.
[Abstract] [Full Text]

Home page
 Genome Res.Home page
K. Ishihara, N. Hatano, H. Furuumi, R. Kato, T. Iwaki, K. Miura, Y. Jinno, and H. Sasaki
Comparative Genomic Sequencing Identifies Novel Tissue-Specific Enhancers and Sequence Elements for Methylation-Sensitive Factors Implicated in Igf2/H19 Imprinting
Genome Res., May 1, 2000; 10(5): 664 - 671.
[Abstract] [Full Text]

Home page
 Physiol. GenomicsHome page
M. B. EINARSON and E. A. GOLEMIS
Encroaching genomics: adapting large-scale science to small academic laboratories
Physiol Genomics, April 27, 2000; 2(3): 85 - 92.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
S. Schwartz, Z. Zhang, K. A. Frazer, A. Smit, C. Riemer, J. Bouck, R. Gibbs, R. Hardison, and W. Miller
PipMaker---A Web Server for Aligning Two Genomic DNA Sequences
Genome Res., April 1, 2000; 10(4): 577 - 586.
[Abstract] [Full Text]

Home page
 Genome Res.Home page
H. M. Robertson
The Large srh Family of Chemoreceptor Genes in Caenorhabditis Nematodes Reveals Processes of Genome Evolution Involving Large Duplications and Deletions and Intron Gains and Losses
Genome Res., February 1, 2000; 10(2): 192 - 203.
[Abstract] [Full Text]

Home page
 Genome Res.Home page
A. Nechiporuk, J. E. Finney, M. T. Keating, and S. L. Johnson
Assessment of Polymorphism in Zebrafish Mapping Strains
Genome Res., December 1, 1999; 9(12): 1231 - 1238.
[Abstract] [Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genes Dev. Learn. Mem.
Protein Science RNA Genome Res.