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Vol. 9, Issue 10, 989-993, October 1999

RESOURCE
A Resource of Mapped Human Bacterial Artificial Chromosome Clones

Vivian G. Cheung,1 Heather L. Dalrymple,1 Sandya Narasimhan,1 Jason Watts,1 Gregory Schuler,2 Anton K. Raap,3 Michael Morley,1 and Alan Bruzel1

1 Department of Pediatrics, The Children's Hospital of Philadelphia, University of Pennsylvania, Philadelphia, Pennsylvania 19104 USA; 2 National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland 20894 USA; 3 Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

To date, despite the increasing number of genomic tools, there is no repository of ordered human BAC clones that covers entire chromosomes. This project presents a resource of mapped large DNA fragments that span eight human chromosomes at ~1-Mb resolution. These DNA fragments are bacterial artificial chromosome (BAC) clones anchored to sequence tagged site (STS) markers. This clone collection, which currently contains 759 mapped clones, is useful in a wide range of applications from microarray-based gene mapping to identification of chromosomal mutations. In addition to the clones themselves, we describe a database, GenMapDB (http://genomics.med.upenn.edu/genmapdb), that contains information about each clone in our collection.

9:989-993 ©1999 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/99 $5.00
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