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Vol. 7, Issue 11, 1085-1093, November 1997

LETTERS
Genetic Mapping of 262 Loci Derived from Expressed Sequences in a Murine Interspecific Cross Using Single-Strand Conformational Polymorphism Analysis

Kevin P. Brady,1 Lucy B. Rowe,2 Helen Her,1 Tamara J. Stevens,3 Janan Eppig,2 Daniel J. Sussman,4 James Sikela,3 and David R. Beier1,5

1 Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115; 2 The Jackson Laboratory, Bar Harbor, Maine 04605; 3 Department of Pharmacology, University of Colorado Health Sciences Center, Denver, Colorado 80262; 4 Division of Human Genetics, University of Maryland School of Medicine, Baltimore, Maryland 21201

We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested using single-strand conformation polymorphism (SSCP) analysis, and that these polymorphisms are useful for genetic mapping. In this report we demonstrate that presumptive 3'-untranslated region sequence obtained from expressed sequence tags (ESTs) can be analyzed in a similar fashion, and we have used this approach to map 262 loci using an interspecific backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of transcribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.

7:1085-1093 ©1997 by Cold Spring Harbor Laboratory Press ISSN 1088-9051/97 $5.00
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