This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rhodes, M Articles by Mundy, C Search for Related Content PubMed PubMed Citation Articles by Rhodes, M Articles by Mundy, C Social Bookmarking                   What's this?

High-throughput microsatellite analysis using fluorescent dUTPs for high-resolution genetic mapping of the mouse genome.

MRC Human Genome Mapping Project Resource Centre, Hinxton, Cambridge, UK.

Abstract

The use of fluorescent end-labeled primers has proved successful for rapid, semiautomated genotyping of microsatellite loci. However, custom synthesis is expensive and costs can be prohibitive when a wide range of markers is to be analyzed for only a few genotypings. This particularly applies to high-resolution genetic mapping in the mouse either in the construction of global maps or in the production of local high-resolution genetic maps for positional cloning. We demonstrate here the use of fluorescent dUTPs for cost-effective, high-throughput microsatellite genotyping in the mouse. This alternative to the use of fluorescent end-labeled primers for semiautomated genotyping is potentially applicable to the construction of linkage maps in other species.

CiteULike

Connotea

Del.icio.us

Digg

Reddit

Technorati

This article has been cited by other articles:

				M. R. De Miglio, F. Canzian, R. M. Pascale, M. M. Simile, M. R. Muroni, D. Calvisi, G. Romeo, and F. Feo Identification of Genetic Loci Controlling Hepatocarcinogenesis on Rat Chromosomes 7 and 10 Cancer Res., September 1, 1999; 59(18): 4651 - 4657. [Abstract] [Full Text] [PDF]

				M. Rhodes, R. Straw, S. Fernando, A. Evans, T. Lacey, A. Dearlove, J. Greystrong, J. Walker, P. Watson, P. Weston, et al. A High-Resolution Microsatellite Map of the Mouse Genome Genome Res., May 1, 1998; 8(5): 531 - 542. [Abstract] [Full Text]