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Published online before print November 9, 2006, 10.1101/gr.5155706
Genome Res. 17:433-440, 2007
©2007 by Cold Spring Harbor Laboratory Press; ISSN 1088-9051/07 $5.00
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Letter

Novel noncoding RNA from human Y distal heterochromatic block (Yq12) generates testis-specific chimeric CDC2L2

Zeenath Jehan1,3, Sambandam Vallinayagam1,3, Shrish Tiwari1, Suman Pradhan1, Lalji Singh1, Amritha Suresh1, Hemakumar M. Reddy1, Y.R. Ahuja2, and Rachel A. Jesudasan1,4

1 Centre for Cellular and Molecular Biology, Uppal Road Hyderabad–500 007, AP, India; 2 Genetics Unit, Vasavi Medical and Research Centre, Hyderabad, India, AP, India

The human Y chromosome, because it is enriched in repetitive DNA, has been very intractable to genetic and molecular analyses. There is no previous evidence for developmental stage- and testis-specific transcription from the male-specific region of the Y (MSY). Here, we present evidence for the first time for a developmental stage- and testis-specific transcription from MSY distal heterochromatic block. We isolated two novel RNAs, which localize to Yq12 in multiple copies, show testis-specific expression, and lack active X-homologs. Experimental evidence shows that one of the above Yq12 noncoding RNAs (ncRNAs) trans-splices with CDC2L2 mRNA from chromosome 1p36.3 locus to generate a testis-specific chimeric beta sv13 isoform. This 67-nt 5'UTR provided by the Yq12 transcript contains within it a Y box protein-binding CCAAT motif, indicating translational regulation of the beta sv13 isoform in testis. This is also the first report of trans-splicing between a Y chromosomal and an autosomal transcript.

3 These two authors contributed equally to this work.

4 Corresponding author.

E-mail rachel{at}ccmb.res.in; fax 91-40-27160311.

[The sequence data from this study have been submitted to GenBank under accession nos. AY598345, AY598346, and AY598347.]

Article published online before print. Article and publication date are at http://www.genome.org/cgi/doi/10.1101/gr.5155706


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