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Methods

DNA Book

¹Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan ²Genome Science Laboratory, RIKEN, Hirosawa, Wako, Saitama, 351-0198, Japan

We propose herein a new method of DNA distribution, whereby DNA clones or PCR products are printed directly onto the pages of books and delivered to users along with relevant scientific information. DNA sheets, comprising water-soluble paper onto which DNA is spotted, can be bound into books. Readers can easily extract the DNA fragments from DNA sheets and amplify them using PCR. We show that DNA sheets can withstand various conditions that may be experienced during bookbinding and delivery, such as high temperatures and humidity. Almost all genes (95%–100% of randomly selected RIKEN mouse cDNA clones) were recovered successfully by use of PCR. Readers can start their experiments after a 2-h PCR amplification without waiting for the delivery of DNA clones. The DNA Book thus provides a novel method for delivering DNA in a timely and cost-effective manner. A sample DNA sheet (carrying RIKEN mouse cDNA clones encoding genes of enzymes for the TCA cycle) is included in this issue for field-testing. We would greatly appreciate it if readers could attempt to extract DNA and report the results and whether the DNA sheet was shipped to readers in good condition.

³ Corresponding authors.
E-MAIL rgscerg{at}gsc.riken.go.jp (for technical issues); FAX 81-45-503-9216.
E-MAIL yosihide{at}gsc.riken.go.jp (for business issues); FAX 81-45-503-9216.

A sample DNA sheet, to which 12 RIKEN cDNA plasmids of TCA cycle genes have been attached, is bound to this article (Fig.3). Clone IDs (DDBJ accession numbers) are 1500012M15 (AK005237), 1500012E04 (AK117103), E030024J03 (AK087063), E430020N12 (AK088582), 9430063I08 (AK034928), 6430550J24 (AK032458), 2610028K08 (AK117104), E230015D12 (AK054053), 1700007H16 (AK005713), 0610009E03 (AK002379), 9430071P19 (AK034998), and E430029P17 (AK088888)

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