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Letter

A Comprehensive Transcript Map of the Mouse Gnas Imprinted Complex

¹The Mammalian Genetics Unit, Medical Research Council, Harwell OX11 ORD, Oxfordshire, UK ²The Institute of Molecular Biosciences, The University of QLD 4072, Brisbane, Australia ³Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, Suehiro-cho, Tsurumi-Ku, Yokohama, Kanagawa, 230-0045, Japan ⁴Genome Science Laboratory, RIKEN, Hirosawa, Wako, Saitama 351-0198, Japan

The recent publication of the FANTOM mouse transcriptome has provided a unique opportunity to study the diversity of transcripts arising from a single gene locus. We have focused on the Gnas complex, as imprinting loci themselves provide unique insights into transcriptional regulation. Thirteen full-length cDNAs from the FANTOM2 set were mapped to the Gnas locus. These represented one previously described transcript and 12 putative new transcripts. Of these, eight were found to be differentially expressed from either the maternal or paternal allele. Two clones extended Nespas in the 3' direction, providing evidence of antisense transcription spanning a 30-kb genomic region from a single allele. The transcripts were summarized into six transcriptional units, Nespas, Nesp, Gnasxl, F7, exon 1A, and Gnas. The resolution of the Gnas transcript map by the FANTOM2 clones revealed a pattern of alternate splicing. In addition to the transcripts described previously as splicing onto exon 2 of Gnas, each new sense transcript had an alternate short 3'UTR independent of Gnas. Both spliced and unspliced variants of the new imprinted sense transcripts were found. Whereas the functional significance of these alternate transcripts is not known, the availability of the FANTOM clones has provided remarkable insights into the repertoire of transcripts in the Gnas complex locus.

⁶ Takahiro Arakawa, Piero Carninci, Jun Kawai, and Yoshihide Hayashizaki.

⁵ Corresponding author.
E-MAIL c.wells{at}imb.uq.edu.au; FAX 61-7-3365488.

[Supplemental material is available online at www.genome.org. The sequence of the clones described in this paper have a RIKEN accession no., and will be assigned GenBank nos. once the data are released.]

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