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METHODS

Screening of Gene-Associated Polymorphisms by Use of In-Gel Competitive Reassociation and EST (cDNA) Array Hybridization

Human Gene 2, Kazusa DNA Research Institute, 2-6-7, Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan

In-gel competitive reassociation (IGCR) is a method of differential subtraction to enrich polymorphic DNA restriction fragments between two DNA samples without probes or specific sequence information. Here, we show that by combining IGCR and expressed sequence tags (EST) array hybridization, polymorphic DNA fragments associated with genes in complex higher organisms (Arabidopsis thaliana) can be effectively screened, demonstrating that this procedure offers a simple and efficient way to obtain gene-associated polymorphic DNA markers.

[The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: N. Sakurai, D. Shibata, and C. Kuwata.]

E-MAIL koshichi{at}kazusa.or.jp; FAX 81-438-52-3946.

Article and publication are at http://www.genome.org/cgi/doi/10.1101/gr.434103.

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