This Article Full Text Full Text (PDF) Supplemental Research Data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, D.-k. Articles by Kim, J.-S. Search for Related Content PubMed PubMed Citation Articles by Lee, D.-k. Articles by Kim, J.-S. Social Bookmarking                   What's this?

Methods

Toward a Functional Annotation of the Human Genome Using Artificial Transcription Factors

ToolGen, Inc., Yuseong-gu, Daejeon, South Korea, 305-390

We have developed a novel, high-throughput approach to collecting randomly perturbed gene-expression profiles from the human genome.A human 293 cell library that stably expresses randomly chosen zinc-finger transcription factors was constructed, and the expression profile of each cell line was obtained using cDNA microarray technology.Gene expression profiles from a total of 132 cell lines were collected and analyzed by (1) a simple clustering method based on expression-profile similarity, and (2) the shortest-path analysis method.These analyses identified a number of gene groups, and further investigation revealed that the genes that were grouped together had close biological relationships.The artificial transcription factor-based random genome perturbation method thus provides a novel functional genomic tool for annotation and classification of genes in the human genome and those of many other organisms.

¹ Corresponding author.
E-MAIL jsk{at}toolgen.com; FAX 82-42-863-3840.

[Supplemental material is available online at www.genome.org. The microarray data from this study have been submitted to GEO under the accession nos.GSM10013-GSM10044 and GSM10069-GSM10168.]

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K.-S. Park, Y.-S. Jang, H. Lee, and J.-S. Kim Phenotypic Alteration and Target Gene Identification Using Combinatorial Libraries of Zinc Finger Proteins in Prokaryotic Cells J. Bacteriol., August 1, 2005; 187(15): 5496 - 5499. [Abstract] [Full Text] [PDF]

				H.-S. Kwon, H.-C. Shin, and J.-S. Kim Suppression of vascular endothelial growth factor expression at the transcriptional and post-transcriptional levels Nucleic Acids Res., April 28, 2005; 33(8): e74 - e74. [Abstract] [Full Text] [PDF]

				P. Blancafort, D. J. Segal, and C. F. Barbas III Designing Transcription Factor Architectures for Drug Discovery Mol. Pharmacol., December 1, 2004; 66(6): 1361 - 1371. [Abstract] [Full Text] [PDF]