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Letter

Characterizing Embryonic Gene Expression Patterns in the Mouse Using Nonredundant Sequence-Based Selection

¹ Division of Mammalian Development, National Institute for Medical Research, The Ridgeway, London NW7 1AA, United Kingdom ² Institute of Molecular Bioscience, University of Queensland, 4072 Australia ³ Unité Génétique Moléculaire Murine, Institut Pasteur, 75015 Paris, France ⁴ Wellcome Trust/Cancer Research UK Institute and Department of Zoology, University of Cambridge, Cambridge CB2 1QR, United Kingdom ⁵ Developmental Biology Program, Victor Chang Cardiac Research Institute, Darlinghurst, 2010, Australia ⁶ Department of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, NSW 2033, Australia

This article investigates the expression patterns of 160 genes that are expressed during early mouse development. The cDNAs were isolated from 7.5 d postcoitum (dpc) endoderm, a region that comprises visceral endoderm (VE), definitive endoderm, and the node–tissues that are required for the initial steps of axial specification and tissue patterning in the mouse. To avoid examining the same gene more than once, and to exclude potentially ubiquitously expressed housekeeping genes, cDNA sequence was derived from 1978 clones of the Endoderm library. These yielded 1440 distinct cDNAs, of which 123 proved to be novel in the mouse. In situ hybridization analysis was carried out on 160 of the cDNAs, and of these, 29 (18%) proved to have restricted expression patterns.

¹⁰ These authors contributed equally to the work described.

⁷ Present address: Wellcome Trust/Cancer Research UK Institute, Cambridge CB2 1QR, UK

⁸ Present address: Institute for Stem Cell Research, The University of Edinburgh, Edinburgh EH9 3JQ, UK.

¹¹ Corresponding authors.
E-MAIL jim{at}welc.cam.ac.uk; FAX 44-1223-33413.
E-MAIL s.dunwoodie{at}victorchang.unsw.edu.au; FAX: 61-02-9295-8501.

⁹ Deceased May 18, 2001.

[Supplemental material is available online at www.genome.org.]

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