Genome Research cityscape

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yuan, Y.
Right arrow Articles by Bennetzen, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yuan, Y.
Right arrow Articles by Bennetzen, J. L.
Right arrowPubmed/NCBI databases
*Nucleotide
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 12, Issue 9, 1345-1349, September 2002

LETTER
Methylation-Spanning Linker Libraries Link Gene-Rich Regions and Identify Epigenetic Boundaries in Zea mays

Yinan Yuan,1 Phillip J. SanMiguel,2 and Jeffrey L. Bennetzen1,3

1 Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA; 2 Genomics Center, Purdue University, West Lafayette, Indiana 47907, USA

Complex cereal genomes are largely composed of small gene-rich regions intermixed with 5 kb to 200 kb blocks of repetitive DNA. The repetitive DNA blocks are usually 5-methylated at 5'-CG-3' and 5'-CNG-3' cytosines in most or all adult tissues, while the genes are generally unmethylated at these sites. We have developed methylation-spanning linker library (MSLL) technology as a tool to span large methylated DNA blocks and thereby link unmethylated genic regions. MSLL clones contain insertions of large fragments that are size fractionated over gels after complete digestion of total genomic DNA with restriction enzymes that are sensitive to the 5-methylation of cytosines in 5'-CG-3' and 5'-CNG-3' sequences. Our data indicate that the end sequences of maize MSLL clones are greatly depleted in repetitive DNAs and enriched in genes relative to total genomic DNA. Combined with other gene-enrichment approaches, MSLL technology can efficiently generate fully-linked contiguous sequences in complex genomes that are resistant to shotgun sequencing.

3 Corresponding author.

12:1345-1349 ©2002 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/02 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
K. M. Devos, J. Ma, A. C. Pontaroli, L. H. Pratt, and J. L. Bennetzen
Analysis and mapping of randomly chosen bacterial artificial chromosome clones from hexaploid bread wheat
PNAS, December 27, 2005; 102(52): 19243 - 19248.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Haberer, S. Young, A. K. Bharti, H. Gundlach, C. Raymond, G. Fuks, E. Butler, R. A. Wing, S. Rounsley, B. Birren, et al.
Structure and Architecture of the Maize Genome
Plant Physiology, December 1, 2005; 139(4): 1612 - 1624.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
J. Emberton, J. Ma, Y. Yuan, P. SanMiguel, and J. L. Bennetzen
Gene enrichment in maize with hypomethylated partial restriction (HMPR) libraries
Genome Res., October 1, 2005; 15(10): 1441 - 1446.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. M. Springer, X. Xu, and W. B. Barbazuk
Utility of Different Gene Enrichment Approaches Toward Identifying and Sequencing the Maize Gene Space
Plant Physiology, October 1, 2004; 136(2): 3023 - 3033.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. J. Sheehan, P. R. Farmer, and T. P. Brutnell
Structure and Expression of Maize Phytochrome Family Homeologs
Genetics, July 1, 2004; 167(3): 1395 - 1405.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genes Dev. Learn. Mem.
Protein Science RNA Genome Res.