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Published online before print July 19, 2002, 10.1101/gr.473902
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Vol. 12, Issue 8, 1231-1245, August 2002

LETTER
Large-Scale Proteomic Analysis of the Human Spliceosome

Juri Rappsilber,1 Ursula Ryder,2 Angus I. Lamond,2 and Matthias Mann1,3

1 Protein Interaction Laboratory in the Center of Experimental Bioinformatics, Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark; 2 Department of Biochemistry, University of Dundee, Dundee DD1 4HN, Scotland, United Kingdom

In a previous proteomic study of the human spliceosome, we identified 42 spliceosome-associated factors, including 19 novel ones. Using enhanced mass spectrometric tools and improved databases, we now report identification of 311 proteins that copurify with splicing complexes assembled on two separate pre-mRNAs. All known essential human splicing factors were found, and 96 novel proteins were identified, of which 55 contain domains directly linking them to functions in splicing/RNA processing. We also detected 20 proteins related to transcription, which indicates a direct connection between this process and splicing. This investigation provides the most detailed inventory of human spliceosome-associated factors to date, and the data indicate a number of interesting links coordinating splicing with other steps in the gene expression pathway.


3 Corresponding author.


12:1231-1245 ©2002 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/02 $5.00

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