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Vol. 12, Issue 2, 222-231, February 2002
Positional Candidate Cloning of a QTL in Dairy Cattle: Identification of a Missense Mutation in the Bovine DGAT1 Gene with Major Effect on Milk Yield and Composition
Bernard
Grisart,1
Wouter
Coppieters,1
Frédéric
Farnir,1
Latifa
Karim,1
Christine
Ford,2
Paulette
Berzi,1
Nadine
Cambisano,1
Myriam
Mni,1
Suzanne
Reid,2
Patricia
Simon,1
Richard
Spelman,3
Michel
Georges,1,4 and
Russell
Snell2
1 Department of Genetics, Faculty of Veterinary Medicine,
University of Liège (B43), 4000-Liège, Belgium;
2 ViaLactia Biosciences (NZ) Ltd., University of Auckland
Medical School, Auckland, New Zealand; 3 Livestock Improvement
Corp., Hamilton, New Zealand
We recently mapped a quantitative trait locus (QTL) with a major
effect on milk composition particularly fat content to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM
chromosome interval bounded by microsatellite markers BULGE13
and BULGE09. We herein report the positional candidate cloning
of this QTL, involving (1) the construction of a BAC contig spanning
the corresponding marker interval, (2) the demonstration that a very
strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene
with a major effect on milk fat content and other milk characteristics.
[The sequence data described in this paper have been submitted to the
GenBank data library under accession number AY065621.]
4
Corresponding author.
12:222-231 ©2002 by Cold Spring Harbor Laboratory Press ISSN 1088-9051/02 $5.00

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