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Vol. 12, Issue 12, 1992-1998, December 2002
METHODS
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  M. Gray, D. I. Shirasaki, C. Cepeda, V. M. Andre, B. Wilburn, X.-H. Lu, J. Tao, I. Yamazaki, S.-H. Li, Y. E. Sun, et al. Full-Length Human Mutant Huntingtin with a Stable Polyglutamine Repeat Can Elicit Progressive and Selective Neuropathogenesis in BACHD Mice J. Neurosci., June 11, 2008; 28(24): 6182 - 6195. [Abstract] [Full Text] [PDF]
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 T. Horsthuis, A. C. Houweling, P. E.M.H. Habets, F. J. de Lange, H. el Azzouzi, D. E.W. Clout, A. F.M. Moorman, and V. M. Christoffels Distinct Regulation of Developmental and Heart Disease-Induced Atrial Natriuretic Factor Expression by Two Separate Distal Sequences Circ. Res., April 11, 2008; 102(7): 849 - 859. [Abstract] [Full Text] [PDF]
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Y. Takashima, R. L. Daniels, W. Knowlton, J. Teng, E. R. Liman, and D. D. McKemy Diversity in the Neural Circuitry of Cold Sensing Revealed by Genetic Axonal Labeling of Transient Receptor Potential Melastatin 8 Neurons J. Neurosci., December 19, 2007; 27(51): 14147 - 14157. [Abstract] [Full Text] [PDF]
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 K. J. T. Venken and H. J. Bellen Transgenesis upgrades for Drosophila melanogaster Development, October 15, 2007; 134(20): 3571 - 3584. [Abstract] [Full Text] [PDF]
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S. Gong, M. Doughty, C. R. Harbaugh, A. Cummins, M. E. Hatten, N. Heintz, and C. R. Gerfen Targeting Cre Recombinase to Specific Neuron Populations with Bacterial Artificial Chromosome Constructs J. Neurosci., September 12, 2007; 27(37): 9817 - 9823. [Abstract] [Full Text] [PDF]
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 Y. Kim, P. Zhou, L. Qian, J.-Z. Chuang, J. Lee, C. Li, C. Iadecola, C. Nathan, and A. Ding MyD88-5 links mitochondria, microtubules, and JNK3 in neurons and regulates neuronal survival J. Exp. Med., September 3, 2007; 204(9): 2063 - 2074. [Abstract] [Full Text] [PDF]
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M. R. Regan, Y. H. Huang, Y. S. Kim, M. I. Dykes-Hoberg, L. Jin, A. M. Watkins, D. E. Bergles, and J. D. Rothstein Variations in Promoter Activity Reveal a Differential Expression and Physiology of Glutamate Transporters by Glia in the Developing and Mature CNS J. Neurosci., June 20, 2007; 27(25): 6607 - 6619. [Abstract] [Full Text] [PDF]
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T. L. Walker, T. Yasuda, D. J. Adams, and P. F. Bartlett The Doublecortin-Expressing Population in the Developing and Adult Brain Contains Multipotential Precursors in Addition to Neuronal-Lineage Cells J. Neurosci., April 4, 2007; 27(14): 3734 - 3742. [Abstract] [Full Text] [PDF]
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 M. I. Kotlikoff Genetically encoded Ca2+ indicators: using genetics and molecular design to understand complex physiology J. Physiol., January 1, 2007; 578(1): 55 - 67. [Abstract] [Full Text] [PDF]
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 C. Thivierge, A. Kurbegovic, M. Couillard, R. Guillaume, O. Cote, and M. Trudel Overexpression of PKD1 Causes Polycystic Kidney Disease Mol. Cell. Biol., February 15, 2006; 26(4): 1538 - 1548. [Abstract] [Full Text] [PDF]
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Y. Jeong, K. El-Jaick, E. Roessler, M. Muenke, and D. J. Epstein A functional screen for sonic hedgehog regulatory elements across a 1 Mb interval identifies long-range ventral forebrain enhancers Development, February 15, 2006; 133(4): 761 - 772. [Abstract] [Full Text] [PDF]
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 L. A. Shakes, D. M. Garland, D. K. Srivastava, K. R. Harewood, and P. K. Chatterjee Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors Nucleic Acids Res., August 1, 2005; 33(13): e118 - e118. [Abstract] [Full Text] [PDF]
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J. P. Segal, N. R. Stallings, C. E. Lee, L. Zhao, N. Socci, A. Viale, T. M. Harris, M. B. Soares, G. Childs, J. K. Elmquist, et al. Use of Laser-Capture Microdissection for the Identification of Marker Genes for the Ventromedial Hypothalamic Nucleus J. Neurosci., April 20, 2005; 25(16): 4181 - 4188. [Abstract] [Full Text] [PDF]
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Q. N. Y. Wong, V. C. W. Ng, M. C. M. Lin, H.-f. Kung, D. Chan, and J.-D. Huang Efficient and seamless DNA recombineering using a thymidylate synthase A selection system in Escherichia coli Nucleic Acids Res., March 30, 2005; 33(6): e59 - e59. [Abstract] [Full Text] [PDF]
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S. Warming, N. Costantino, D. L. Court, N. A. Jenkins, and N. G. Copeland Simple and highly efficient BAC recombineering using galK selection Nucleic Acids Res., February 24, 2005; 33(4): e36 - e36. [Abstract] [Full Text] [PDF]
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P. K. Chatterjee, L. A. Shakes, D. K. Srivastava, D. M. Garland, K. R. Harewood, K. J. Moore, and J. S. Coren Mutually exclusive recombination of wild-type and mutant loxP sites in vivo facilitates transposon-mediated deletions from both ends of genomic DNA in PACs Nucleic Acids Res., October 19, 2004; 32(18): 5668 - 5676. [Abstract] [Full Text] [PDF]
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Origin of Replication
origin for DNA replication,
the E. coli RecA gene for recombination, and the SacB
gene for negative selection. These new vectors greatly increased the
ease with which one can clone the shuttle vectors, as well as screen
for co-integrated and resolved clones. Furthermore, we simplify the
shuttle vector cloning to one step by incorporation of a "built-in"
resolution cassette for rapid removal of the unwanted vector sequences.
This new system has been used to modify a dozen BACs. It is well suited
for efficient production of modified BACs for use in a variety of in
vivo studies.