Genome Research International Con Genetics

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Research Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stickney, H. L.
Right arrow Articles by Talbot, W. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stickney, H. L.
Right arrow Articles by Talbot, W. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 12, Issue 12, 1929-1934, December 2002

METHODS
Rapid Mapping of Zebrafish Mutations With SNPs and Oligonucleotide Microarrays

Heather L. Stickney,1 Jeremy Schmutz,2 Ian G. Woods,1 Caleb C. Holtzer,2 Mark C. Dickson,2 Peter D. Kelly,1 Richard M. Myers,2 and William S. Talbot1,3

1 Department of Developmental Biology, Stanford University, Stanford, California 94305, USA; 2 Department of Genetics and the Stanford Human Genome Center, Stanford University, Stanford, California 94305, USA

Large-scale genetic screens in zebrafish have identified thousands of mutations in hundreds of essential genes. The genetic mapping of these mutations is necessary to link DNA sequences to the gene functions defined by mutant phenotypes. Here, we report two advances that will accelerate the mapping of zebrafish mutations: (1) The construction of a first generation single nucleotide polymorphism (SNP) map of the zebrafish genome comprising 2035 SNPs and 178 small insertions/deletions, and (2) the development of a method for mapping mutations in which hundreds of SNPs can be scored in parallel with an oligonucleotide microarray. We have demonstrated the utility of the microarray technique in crosses with haploid and diploid embryos by mapping two known mutations to their previously identified locations. We have also used this approach to localize four previously unmapped mutations. We expect that mapping with SNPs and oligonucleotide microarrays will accelerate the molecular analysis of zebrafish mutations.

[Supplemental material is available online at www.genome.org. The sequence data described in this paper have been submitted to dbSNP under accession nos. 5103507-5105537. The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: J. Postlethwait, C.-B. Chien, C. Kimmel, L. Maves, and M. Westerfield.]

3 Corresponding author.

12:1929-1934 ©2002 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/02 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Clin. Chem.Home page
K. Boissinot, A. Huletsky, R. Peytavi, S. Turcotte, V. Veillette, M. Boissinot, F. J. Picard, E. A. Martel, and M. G. Bergeron
Rapid exonuclease digestion of pcr-amplified targets for improved microarray hybridization.
Clin. Chem., November 1, 2007; 53(11): 2020 - 2023.
[Full Text] [PDF]

Home page
 Genome Res.Home page
V. Guryev, M. J. Koudijs, E. Berezikov, S. L. Johnson, R. H.A. Plasterk, F. J.M. van Eeden, and E. Cuppen
Genetic variation in the zebrafish
Genome Res., April 1, 2006; 16(4): 491 - 497.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Pietsch, J.-M. Delalande, B. Jakaitis, J. D. Stensby, S. Dohle, W. S. Talbot, D. W. Raible, and I. T. Shepherd
lessen encodes a zebrafish trap100 required for enteric nervous system development
Development, February 1, 2006; 133(3): 395 - 406.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
H. Roest Crollius and J. Weissenbach
Fish genomics and biology
Genome Res., December 1, 2005; 15(12): 1675 - 1682.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Ruparel, L. Bi, Z. Li, X. Bai, D. H. Kim, N. J. Turro, and J. Ju
Design and synthesis of a 3'-O-allyl photocleavable fluorescent nucleotide as a reversible terminator for DNA sequencing by synthesis
PNAS, April 26, 2005; 102(17): 5932 - 5937.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
C. F. Crane and Y. M. Crane
A nearest-neighboring-end algorithm for genetic mapping
Bioinformatics, April 15, 2005; 21(8): 1579 - 1591.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. A. Yoder, R. T. Litman, M. G. Mueller, S. Desai, K. P. Dobrinski, J. S. Montgomery, M. P. Buzzeo, T. Ota, C. T. Amemiya, N. S. Trede, et al.
Resolution of the novel immune-type receptor gene cluster in zebrafish
PNAS, November 2, 2004; 101(44): 15706 - 15711.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
H. Ruparel, M. E. Ulz, S. Kim, and J. Ju
Digital Detection of Genetic Mutations Using SPC-Sequencing
Genome Res., February 1, 2004; 14(2): 296 - 300.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
A. Amores, T. Suzuki, Y.-L. Yan, J. Pomeroy, A. Singer, C. Amemiya, and J. H. Postlethwait
Developmental Roles of Pufferfish Hox Clusters and Genome Evolution in Ray-Fin Fish
Genome Res., January 1, 2004; 14(1): 1 - 10.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genes Dev. Learn. Mem.
Protein Science RNA Genome Res.