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Published online before print December 14, 2001, 10.1101/gr.181301
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Vol. 12, Issue 1, 132-144, January 2002

LETTER
Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNA

Catherine Cloix,1 Sylvie Tutois,1 Yasushi Yukawa,2 Olivier Mathieu,1 Claudine Cuvillier,1 Marie-Claude Espagnol,1 Georges Picard,1 and Sylvette Tourmente1,3

1 U.M.R. 6547 BIOMOVE, Université Blaise Pascal, 24 Avenue des Landais, 63177 Aubière Cedex, France; 2 Center for Gene Research, Nagoya University, Nagoya 464- 8602, Japan

One major 5S RNA, 120 bases long, was revealed by an analysis of mature 5S RNA from tissues, developmental stages, and polysomes in Arabidopsis thaliana. Minor 5S RNA were also found, varying from the major one by one or two base substitutions; 5S rDNA units from each 5S array of the Arabidopsis genome were isolated by PCR using CIC yeast artificial chromosomes (YACs) mapped on the different loci. By using a comparison of the 5S DNA and RNA sequences, we could show that both major and minor 5S transcripts come from only two of the genomic 5S loci: chromosome 4 and chromosome 5 major block. Other 5S loci are either not transcribed or produce rapidly degraded 5S transcripts. Analysis of the 5'- and 3'-DNA flanking sequence has permitted the definition of specific signatures for each 5S rDNA array.

[EMBL accession nos: AF330825-AF331032; AF335777-AF335873.]

3 Corresponding author.

12:132-144 ©2002 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/02 $5.00
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