Vol. 12, Issue 1, 112-121, January 2002

LETTER
Expression Profiles of 290 ESTs Mapped to Chromosome 3 in Human Epithelial Ovarian Cancer Cell Lines Using DNA Expression Oligonucleotide Microarrays

¹ Department of Human Genetics, McGill University, Montreal, Quebec H3A 1B1, Canada; ² Centre de recherche du Centre Hospitalier de l'Université de Montréal (CHUM)-Hôpital Notre-Dame and Institut du cancer de Montréal, Montreal, Quebec H2L 4M1, Canada; ³ Département de médecine, et ⁴ Département d'obstétrique-gynécologie, Université de Montréal, Montreal, Quebec H3C 3J7, Canada; ⁵ Montreal Genome Centre, McGill University Health Centre, Montreal, Quebec H3G 1A4, Canada; ⁶ Department of Medicine, McGill University, Montreal, Quebec H3G 1A4, Canada; ⁷ Research Institute, McGill University Health Centre, Montreal General Hospital, Montreal, Quebec H3G 1A4, Canada.

We have investigated previously the utility of oligonucleotide expression microarray technology in an analysis of four spontaneously transformed epithelial ovarian cancer (EOC) cell lines, TOV-21G, TOV-81D, OV-90, and TOV-112D. Here, we examine the expression of 290 expressed sequence tags (ESTs) that map to human chromosome 3 in a primary culture derived from normal ovarian surface epithelium (NOSE), NOV-31, and the four spontaneously transformed EOC cell lines. One of these cell lines, OV-90, harbors a deletion of an entire chromosome 3p arm. Whereas the most aggressive cell lines (OV-90, TOV-112D, and TOV-21G) exhibited the highest levels of expression, assessed by the mean of expression values of all ESTs, OV-90 showed the lowest mean of expression of ESTs that map to the 3p arm in comparison with TOV-112D and TOV-21G. This difference in expression profile of 3p ESTs in OV-90 is also reflected in the ratio of expression of ESTs on 3p versus the 3q arm and in that the expression values of ESTs that map to 3p were more often lower than higher in OV-90 in two-way comparisons with NOV-31, TOV-21G, and TOV-112D. The loss of a 3p arm does not affect the pattern of differential expression in analyses based on the range of numeric expression values of each EST or fold differences in expression for each EST in comparison with NOV-31. However, 25 differentially expressed ESTs were identified on the basis of threefold differences in expression values between NOV-31 and any EOC cell line; and six of these ESTs were differentially expressed uniquely in OV-90. The investigation of these ESTs could facilitate the identification of novel chromosome 3 genes implicated in ovarian tumorigenesis.