Genome Research cityscape

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bolivar, V. J.
Right arrow Articles by Flaherty, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bolivar, V. J.
Right arrow Articles by Flaherty, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 11, Issue 9, 1549-1552, September 2001

METHODS
Mapping of Quantitative Trait Loci with Knockout/Congenic Strains

Valerie J. Bolivar, Melloni N. Cook, and Lorraine Flaherty1

Genomics Institute, Wadsworth Center, Albany 12201-2002, New York, USA, Department of Pediatrics, Albany Medical College, Albany 12201, New York, USA; Department of Biomedical Sciences, University at Albany, Albany 12201, New York, USA

Recently we have explored the use of knockout/congenic mouse strains for isolating and mapping quantitative trait loci (QTLs). Because most knockout strains have been bred to be B6.129 congenic strains, they can be used to test for QTLs in the targeted chromosomal area as long as there is a genetic difference between B6 and 129. Thus, we have tested a number of knockout/congenic strains in a series of behavioral tests in which mouse performance has a significant genetic component. We have also developed a breeding scheme for distinguishing the effects of background flanking genes from the targeted ablation. In screening several knockout/congenics, we have found at least one that harbors a behavioral QTL in the 129 chromosomal segment. The position of this QTL was confirmed subsequently by several F1 crosses.

1 Corresponding author.

11:1549-1552 ©2001 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/01 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
L. Verret, J. L. Jankowsky, G. M. Xu, D. R. Borchelt, and C. Rampon
Alzheimer's-Type Amyloidosis in Transgenic Mice Impairs Survival of Newborn Neurons Derived from Adult Hippocampal Neurogenesis
J. Neurosci., June 20, 2007; 27(25): 6771 - 6780.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. A. Perez and R. D. Palmiter
Parkin-deficient mice are not a robust model of parkinsonism
PNAS, February 8, 2005; 102(6): 2174 - 2179.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Xu, P. A. Koni, P. Wang, G. Li, L. Kaczmarek, Y. Wu, Y. Li, R. A. Flavell, and G. V. Desir
The voltage-gated potassium channel Kv1.3 regulates energy homeostasis and body weight
Hum. Mol. Genet., March 1, 2003; 12(5): 551 - 559.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
J. Peirce
Looking at Old Tools in New Ways: Using Knockouts as Congenics to Study QTLs
Genome Res., September 1, 2001; 11(9): 1469 - 1471.
[Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genes Dev. Learn. Mem.
Protein Science RNA Genome Res.