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Published online before print June 12, 2001, 10.1101/gr.GR-1748R
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Vol. 11, Issue 7, 1256-1261, July 2001

METHODS
Arrays of Arrays for High-Throughput Gene Expression Profiling

Patrick P. Zarrinkar,1,3,5,6 James K. Mainquist,1 Matthew Zamora,1 David Stern,2 John B. Welsh,1 Lisa M. Sapinoso,1 Garret M. Hampton,1,5,7 and David J. Lockhart1,2,3,4,5,8

1 Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, USA; 2 Affymetrix, Inc., Santa Clara, California 95051, USA

Gene expression profiling using DNA arrays is rapidly becoming an essential tool for research and drug discovery and may soon play a central role in disease diagnosis. Although it is possible to make significant discoveries on the basis of a relatively small number of expression profiles, the full potential of this technology is best realized through more extensive collections of expression measurements. The generation of large numbers of expression profiles can be a time-consuming and labor-intensive process with current one-at-a-time technology. We have developed the ability to obtain expression profiles in a highly parallel yet straightforward format using glass wafers that contain 49 individual high-density oligonucleotide arrays. This arrays of arrays concept is generalizable and can be adapted readily to other types of arrays, including spotted cDNA microarrays. It is also scalable for use with hundreds and even thousands of smaller arrays on a single piece of glass. Using the arrays of arrays approach and parallel preparation of hybridization samples in 96-well plates, we were able to determine the patterns of gene expression in 27 ovarian carcinomas and 4 normal ovarian tissue samples, along with a number of control samples, in a single experiment. This new approach significantly increases the ease, efficiency, and throughput of microarray-based experiments and makes possible new applications of expression profiling that are currently impractical.

Present address: 3Aventa Biosciences Corp., San Diego, California 92121, USA; 4The Salk Institute for Biological Studies, 10010 No. Torrey Pines Road, La Jolla, CA 92037, USA.

5 Corresponding authors.

6 E-MAIL pzarrinkar{at}aventabio.com; FAX (858) 458-4599.

7 E-MAIL hampton{at}gnf.org; FAX (858) 812-1570.

8 E-MAIL davidlockhart_2000{at}yahoo.com; FAX (858) 458-4599.

11:1256-1261 ©2001 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/01 $5.00
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