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Vol. 11, Issue 7, 1246-1255, July 2001

METHODS
Phenotype MicroArrays for High-Throughput Phenotypic Testing and Assay of Gene Function

Barry R. Bochner,1 Peter Gadzinski, and Eugenia Panomitros

Biolog, Inc., Hayward, California 94545, USA

The bacterium Escherichia coli is used as a model cellular system to test and validate a new technology called Phenotype MicroArrays (PMs). PM technology is a high-throughput technology for simultaneous testing of a large number of cellular phenotypes. It consists of preconfigured well arrays in which each well tests a different cellular phenotype and an automated instrument that continuously monitors and records the response of the cells in all wells of the arrays. For example, nearly 700 phenotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays. PMs can be used to directly assay the effects of genetic changes on cells, especially gene knock-outs. Here, we provide data on phenotypic analysis of six strains and show that we can detect expected phenotypes as well as, in some cases, unexpected phenotypes.

1 Corresponding author.

11:1246-1255 ©2001 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/01 $5.00
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