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Vol. 11, Issue 5, 850-862, May 2001

LETTER
Human-Ovine Comparative Sequencing of a 250-kb Imprinted Domain Encompassing the Callipyge (clpg) Locus and Identification of Six Imprinted Transcripts: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8

Carole Charlier,1 Karin Segers,1 Danny Wagenaar,1 Latifa Karim,1 Stéphane Berghmans,1 Olivier Jaillon,2 Tracy Shay,3 Jean Weissenbach,2 Noelle Cockett,3 Gabor Gyapay,2 and Michel Georges1,4

1 Department of Genetics, Faculty of Veterinary Medicine, University of Liège (B43), 4000-Liège, Belgium; 2 Genoscope, Centre National de Séquençage, CP 5706, 91057 EVRY Cedex, France; 3 Department of Animal, Dairy, and Veterinary Sciences, College of Agriculture, Utah State University, Logan, Utah 84322-4700, USA

Two ovine BAC clones and a connecting long-range PCR product, jointly spanning ~250 kb and representing most of the MULGE5-OY3 marker interval known to contain the clpg locus, were completely sequenced. The resulting genomic sequence was aligned with its human ortholog and extensively annotated. Six transcripts, four of which were novel, were predicted to originate from within the analyzed region and their existence confirmed experimentally: DLK1, DAT, GTL2, PEG11, antiPEG11, and MEG8. RT-PCR experiments performed on a range of tissues sampled from an 8-wk-old animal demonstrated the preferential expression of all six transcripts in skeletal muscle, which suggests that they are under control of common regulatory elements. The six transcripts were also shown to be subject to parental imprinting: DLK1, DAT, and PEG11 were shown to be paternally expressed and GTL2, antiPEG11, and MEG8 to be maternally expressed.

[The sequence data described in this paper have been submitted to the GenBank data library under accession no. AF354168.]

4 Corresponding author.

11:850-862 ©2001 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/01 $5.00
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