Genome Research International Con Genetics

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gordon, D.
Right arrow Articles by Green, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gordon, D.
Right arrow Articles by Green, P.
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 11, Issue 4, 614-625, April 2001

METHODS
Automated Finishing with Autofinish

David Gordon,1,3 Cindy Desmarais,1 and Phil Green1,2

1 Department of Molecular Biotechnology, University of Washington, Seattle, Washington 98195, USA; 2 Howard Hughes Medical Institute, Chevy Chase, Maryland 20815, USA

Currently, the genome sequencing community is producing shotgun sequence data at a very high rate, but finishing (collecting additional directed sequence data to close gaps and improve the quality of the data) is not matching that rate. One reason for the difference is that shotgun sequencing is highly automated but finishing is not: Most finishing decisions, such as which directed reads to obtain and which specialized sequencing techniques to use, are made by people. If finishing rates are to increase to match shotgun sequencing rates, most finishing decisions also must be automated. The Autofinish computer program (which is part of the Consed computer software package) does this by automatically choosing finishing reads. Autofinish is able to suggest most finishing reads required for completion of each sequencing project, greatly reducing the amount of human attention needed. Autofinish sometimes completely finishes the project, with no human decisions required. It cannot solve the most complex problems, so we recommend that Autofinish be allowed to suggest reads for the first three rounds of finishing, and if the project still is not finished completely, a human finisher complete the work. We compared this Autofinish-Hybrid method of finishing against a human finisher in five different projects with a variety of shotgun depths by finishing each project twice---once with each method. This comparison shows that the Autofinish-Hybrid method saves many hours over a human finisher alone, while using roughly the same number and type of reads and closing gaps at roughly the same rate. Autofinish currently is in production use at several large sequencing centers. It is designed to be adaptable to the finishing strategy of the lab---it can finish using some or all of the following: resequencing reads, reverses, custom primer walks on either subclone templates or whole clone templates, PCR, or minilibraries. Autofinish has been used for finishing cDNA, genomic clones, and whole bacterial genomes (see http://www.phrap.org).


3 Corresponding author.


11:614-625 ©2001 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/01 $5.00

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
DNA ResHome page
M. Naito, H. Hirakawa, A. Yamashita, N. Ohara, M. Shoji, H. Yukitake, K. Nakayama, H. Toh, F. Yoshimura, S. Kuhara, et al.
Determination of the Genome Sequence of Porphyromonas gingivalis Strain ATCC 33277 and Genomic Comparison with Strain W83 Revealed Extensive Genome Rearrangements in P. gingivalis
DNA Res, June 3, 2008; (2008) dsn013v1.
[Abstract] [Full Text] [PDF]


Home page
DNA ResHome page
H. Morita, H. Toh, S. Fukuda, H. Horikawa, K. Oshima, T. Suzuki, M. Murakami, S. Hisamatsu, Y. Kato, T. Takizawa, et al.
Comparative Genome Analysis of Lactobacillus reuteri and Lactobacillus fermentum Reveal a Genomic Island for Reuterin and Cobalamin Production
DNA Res, May 16, 2008; (2008) dsn009v1.
[Abstract] [Full Text] [PDF]


Home page
Mol Biol EvolHome page
N. Warthmann, S. Das, C. Lanz, and D. Weigel
Comparative Analysis of the MIR319a MicroRNA Locus in Arabidopsis and Related Brassicaceae
Mol. Biol. Evol., May 1, 2008; 25(5): 892 - 902.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
K.-H. Gartemann, B. Abt, T. Bekel, A. Burger, J. Engemann, M. Flugel, L. Gaigalat, A. Goesmann, I. Grafen, J. Kalinowski, et al.
The Genome Sequence of the Tomato-Pathogenic Actinomycete Clavibacter michiganensis subsp. michiganensis NCPPB382 Reveals a Large Island Involved in Pathogenicity
J. Bacteriol., March 15, 2008; 190(6): 2138 - 2149.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
J. F. Storz, F. G. Hoffmann, J. C. Opazo, and H. Moriyama
Adaptive Functional Divergence Among Triplicated {alpha}-Globin Genes in Rodents
Genetics, March 1, 2008; 178(3): 1623 - 1638.
[Abstract] [Full Text] [PDF]


Home page
DNA ResHome page
T. Goto, A. Yamashita, H. Hirakawa, M. Matsutani, K. Todo, K. Ohshima, H. Toh, K. Miyamoto, S. Kuhara, M. Hattori, et al.
Complete Genome Sequence of Finegoldia magna, an Anaerobic Opportunistic Pathogen
DNA Res, February 7, 2008; (2008) dsm030v1.
[Abstract] [Full Text] [PDF]


Home page
CirculationHome page
C. M. Albert, E. G. Nam, E. B. Rimm, H. W. Jin, R. J. Hajjar, D. J. Hunter, C. A. MacRae, and P. T. Ellinor
Cardiac Sodium Channel Gene Variants and Sudden Cardiac Death in Women
Circulation, January 1, 2008; 117(1): 16 - 23.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
R. Sorek, Y. Zhu, C. J. Creevey, M. P. Francino, P. Bork, and E. M. Rubin
Genome-Wide Experimental Determination of Barriers to Horizontal Gene Transfer
Science, November 30, 2007; 318(5855): 1449 - 1452.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
A. Schluter, I. Krahn, F. Kollin, G. Bonemann, M. Stiens, R. Szczepanowski, S. Schneiker, and A. Puhler
IncP-1{beta} Plasmid pGNB1 Isolated from a Bacterial Community from a Wastewater Treatment Plant Mediates Decolorization of Triphenylmethane Dyes
Appl. Envir. Microbiol., October 15, 2007; 73(20): 6345 - 6350.
[Abstract] [Full Text] [PDF]


Home page
J HeredHome page
N. Yuhki, T. Beck, R. Stephens, B. Neelam, and S. J. O'Brien
Comparative Genomic Structure of Human, Dog, and Cat MHC: HLA, DLA, and FLA
J. Hered., August 3, 2007; (2007) esm056v1.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
A. Schluter, R. Szczepanowski, N. Kurz, S. Schneiker, I. Krahn, and A. Puhler
Erythromycin Resistance-Conferring Plasmid pRSB105, Isolated from a Sewage Treatment Plant, Harbors a New Macrolide Resistance Determinant, an Integron-Containing Tn402-Like Element, and a Large Region of Unknown Function
Appl. Envir. Microbiol., March 15, 2007; 73(6): 1952 - 1960.
[Abstract] [Full Text] [PDF]


Home page
Antimicrob. Agents Chemother.Home page
R. Szczepanowski, I. Krahn, N. Bohn, A. Puhler, and A. Schluter
Novel Macrolide Resistance Module Carried by the IncP-1{beta} Resistance Plasmid pRSB111, Isolated from a Wastewater Treatment Plant
Antimicrob. Agents Chemother., February 1, 2007; 51(2): 673 - 678.
[Abstract] [Full Text] [PDF]


Home page
Infect. Immun.Home page
L. Rohmer, M. Brittnacher, K. Svensson, D. Buckley, E. Haugen, Y. Zhou, J. Chang, R. Levy, H. Hayden, M. Forsman, et al.
Potential Source of Francisella tularensis Live Vaccine Strain Attenuation Determined by Genome Comparison
Infect. Immun., December 1, 2006; 74(12): 6895 - 6906.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
T. Hain, C. Steinweg, C. T. Kuenne, A. Billion, R. Ghai, S. S. Chatterjee, E. Domann, U. Karst, A. Goesmann, T. Bekel, et al.
Whole-Genome Sequence of Listeria welshimeri Reveals Common Steps in Genome Reduction with Listeria innocua as Compared to Listeria monocytogenes
J. Bacteriol., November 1, 2006; 188(21): 7405 - 7415.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
K. Smalla, A. S. Haines, K. Jones, E. Krogerrecklenfort, H. Heuer, M. Schloter, and C. M. Thomas
Increased Abundance of IncP-1{beta} Plasmids and Mercury Resistance Genes in Mercury-Polluted River Sediments: First Discovery of IncP-1{beta} Plasmids with a Complex mer Transposon as the Sole Accessory Element
Appl. Envir. Microbiol., November 1, 2006; 72(11): 7253 - 7259.
[Abstract] [Full Text] [PDF]


Home page
RNAHome page
M. Stapleton, J. W. Carlson, and S. E. Celniker
RNA editing in Drosophila melanogaster: New targets and functional consequences
RNA, November 1, 2006; 12(11): 1922 - 1932.
[Abstract] [Full Text] [PDF]


Home page
Mol Hum ReprodHome page
A. Dokras, J. Coffin, L. Field, A. Frakes, H. Lee, A. Madan, T. Nelson, G.-Y. Ryu, J.-G. Yoon, and A. Madan
Epigenetic regulation of maspin expression in the human placenta
Mol. Hum. Reprod., October 1, 2006; 12(10): 611 - 617.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
D. M. Bulach, R. L. Zuerner, P. Wilson, T. Seemann, A. McGrath, P. A. Cullen, J. Davis, M. Johnson, E. Kuczek, D. P. Alt, et al.
Genome reduction in Leptospira borgpetersenii reflects limited transmission potential
PNAS, September 26, 2006; 103(39): 14560 - 14565.
[Abstract] [Full Text] [PDF]


Home page
Antimicrob. Agents Chemother.Home page
G. Bonemann, M. Stiens, A. Puhler, and A. Schluter
Mobilizable IncQ-Related Plasmid Carrying a New Quinolone Resistance Gene, qnrS2, Isolated from the Bacterial Community of a Wastewater Treatment Plant.
Antimicrob. Agents Chemother., September 1, 2006; 50(9): 3075 - 3080.
[Abstract] [Full Text] [PDF]


Home page
J. Virol.Home page
V. M. Deyde, M. L. Khristova, P. E. Rollin, T. G. Ksiazek, and S. T. Nichol
Crimean-congo hemorrhagic Fever virus genomics and global diversity.
J. Virol., September 1, 2006; 80(17): 8834 - 8842.
[Abstract] [Full Text] [PDF]


Home page
MicrobiologyHome page
A. S. Haines, P. Akhtar, E. R. Stephens, K. Jones, C. M. Thomas, C. D. Perkins, J. R. Williams, M. J. Day, and J. C. Fry
Plasmids from freshwater environments capable of IncQ retrotransfer are diverse and include pQKH54, a new IncP-1 subgroup archetype.
Microbiology, September 1, 2006; 152(Pt 9): 2689 - 2701.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
G. Foltz, G.-Y. Ryu, J.-G. Yoon, T. Nelson, J. Fahey, A. Frakes, H. Lee, L. Field, K. Zander, Z. Sibenaller, et al.
Genome-Wide Analysis of Epigenetic Silencing Identifies BEX1 and BEX2 as Candidate Tumor Suppressor Genes in Malignant Glioma.
Cancer Res., July 1, 2006; 66(13): 6665 - 6674.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
S. L. Dellaporta, A. Xu, S. Sagasser, W. Jakob, M. A. Moreno, L. W. Buss, and B. Schierwater
Mitochondrial genome of Trichoplax adhaerens supports Placozoa as the basal lower metazoan phylum
PNAS, June 6, 2006; 103(23): 8751 - 8756.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
M. Stiens, S. Schneiker, M. Keller, S. Kuhn, A. Puhler, and A. Schluter
Sequence Analysis of the 144-Kilobase Accessory Plasmid pSmeSM11a, Isolated from a Dominant Sinorhizobium meliloti Strain Identified during a Long-Term Field Release Experiment.
Appl. Envir. Microbiol., May 1, 2006; 72(5): 3662 - 3672.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
M. Gaillard, T. Vallaeys, F. J. Vorholter, M. Minoia, C. Werlen, V. Sentchilo, A. Puhler, and J. R. van der Meer
The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties.
J. Bacteriol., March 1, 2006; 188(5): 1999 - 2013.
[Abstract] [Full Text] [PDF]


Home page
Genome Res.Home page
H. Toh, B. L. Weiss, S. A.H. Perkin, A. Yamashita, K. Oshima, M. Hattori, and S. Aksoy
Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host
Genome Res., February 1, 2006; 16(2): 149 - 156.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
P. F. Cliften, R. S. Fulton, R. K. Wilson, and M. Johnston
After the Duplication: Gene Loss and Adaptation in Saccharomyces Genomes
Genetics, February 1, 2006; 172(2): 863 - 872.
[Abstract] [Full Text] [PDF]


Home page
DNA ResHome page
Y. Azuma, H. Hirakawa, A. Yamashita, Y. Cai, M. A. Rahman, H. Suzuki, S. Mitaku, H. Toh, S. Goto, T. Murakami, et al.
Genome Sequence of the Cat Pathogen, Chlamydophila felis
DNA Res, January 1, 2006; 13(1): 15 - 23.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
R. A. Hoskins, M. Stapleton, R. A. George, C. Yu, K. H. Wan, J. W. Carlson, and S. E. Celniker
Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)
Nucleic Acids Res., December 2, 2005; 33(21): e185 - e185.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
K. V. Krutovsky and D. B. Neale
Nucleotide Diversity and Linkage Disequilibrium in Cold-Hardiness- and Wood Quality-Related Candidate Genes in Douglas Fir
Genetics, December 1, 2005; 171(4): 2029 - 2041.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
F. Thieme, R. Koebnik, T. Bekel, C. Berger, J. Boch, D. Buttner, C. Caldana, L. Gaigalat, A. Goesmann, S. Kay, et al.
Insights into Genome Plasticity and Pathogenicity of the Plant Pathogenic Bacterium Xanthomonas campestris pv. vesicatoria Revealed by the Complete Genome Sequence
J. Bacteriol., November 1, 2005; 187(21): 7254 - 7266.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
S. N. Gardner, M. W. Lam, J. R. Smith, C. L. Torres, and T. R. Slezak
Draft versus finished sequence data for DNA and protein diagnostic signature development
Nucleic Acids Res., October 20, 2005; 33(18): 5838 - 5850.
[Abstract] [Full Text] [PDF]


Home page
J. Gen. Virol.Home page
A. M. Likos, S. A. Sammons, V. A. Olson, A. M. Frace, Y. Li, M. Olsen-Rasmussen, W. Davidson, R. Galloway, M. L. Khristova, M. G. Reynolds, et al.
A tale of two clades: monkeypox viruses
J. Gen. Virol., October 1, 2005; 86(10): 2661 - 2672.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
M. Kuroda, A. Yamashita, H. Hirakawa, M. Kumano, K. Morikawa, M. Higashide, A. Maruyama, Y. Inose, K. Matoba, H. Toh, et al.
Whole genome sequence of Staphylococcus saprophyticus reveals the pathogenesis of uncomplicated urinary tract infection
PNAS, September 13, 2005; 102(37): 13272 - 13277.
[Abstract] [Full Text] [PDF]


Home page
Appl. Environ. Microbiol.Home page
S. Reslewic, S. Zhou, M. Place, Y. Zhang, A. Briska, S. Goldstein, C. Churas, R. Runnheim, D. Forrest, A. Lim, et al.
Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum
Appl. Envir. Microbiol., September 1, 2005; 71(9): 5511 - 5522.
[Abstract] [Full Text] [PDF]


Home page
Genome Res.Home page
C. K. Raymond, A. Kas, M. Paddock, R. Qiu, Y. Zhou, S. Subramanian, J. Chang, A. Palmieri, E. Haugen, R. Kaul, et al.
Ancient haplotypes of the HLA Class II region
Genome Res., September 1, 2005; 15(9): 1250 - 1257.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
A. S. Haines, K. Jones, M. Cheung, and C. M. Thomas
The IncP-6 Plasmid Rms149 Consists of a Small Mobilizable Backbone with Multiple Large Insertions
J. Bacteriol., July 15, 2005; 187(14): 4728 - 4738.
[Abstract] [Full Text] [PDF]


Home page
Genome Res.Home page
B. Negre, S. Casillas, M. Suzanne, E. Sanchez-Herrero, M. Akam, M. Nefedov, A. Barbadilla, P. de Jong, and A. Ruiz
Conservation of regulatory sequences and gene expression patterns in the disintegrating Drosophila Hox gene complex
Genome Res., May 1, 2005; 15(5): 692 - 700.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
E. E. Smith, E. H. Sims, D. H. Spencer, R. Kaul, and M. V. Olson
Evidence for Diversifying Selection at the Pyoverdine Locus of Pseudomonas aeruginosa
J. Bacteriol., March 15, 2005; 187(6): 2138 - 2147.
[Abstract] [Full Text] [PDF]


Home page
Genome Res.Home page
R. W. Blakesley, N. F. Hansen, J. C. Mullikin, P. J. Thomas, J. C. McDowell, B. Maskeri, A. C. Young, B. Benjamin, S. Y. Brooks, B. I. Coleman, et al.
An intermediate grade of finished genomic sequence suitable for comparative analyses
Genome Res., November 1, 2004; 14(11): 2235 - 2244.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
E. L. Hendrickson, R. Kaul, Y. Zhou, D. Bovee, P. Chapman, J. Chung, E. Conway de Macario, J. A. Dodsworth, W. Gillett, D. E. Graham, et al.
Complete Genome Sequence of the Genetically Tractable Hydrogenotrophic Methanogen Methanococcus maripaludis
J. Bacteriol., October 15, 2004; 186(20): 6956 - 6969.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
J. Ishikawa, A. Yamashita, Y. Mikami, Y. Hoshino, H. Kurita, K. Hotta, T. Shiba, and M. Hattori
The complete genomic sequence of Nocardia farcinica IFM 10152
PNAS, October 12, 2004; 101(41): 14925 - 14930.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
K. Ueda, A. Yamashita, J. Ishikawa, M. Shimada, T.-o Watsuji, K. Morimura, H. Ikeda, M. Hattori, and T. Beppu
Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism
Nucleic Acids Res., September 21, 2004; 32(16): 4937 - 4944.
[Abstract] [Full Text] [PDF]


Home page
GeneticsHome page
K. V. Krutovsky, M. Troggio, G. R. Brown, K. D. Jermstad, and D. B. Neale
Comparative Mapping in the Pinaceae
Genetics, September 1, 2004; 168(1): 447 - 461.
[Abstract] [Full Text] [PDF]


Home page
Plant Physiol.Home page
J. Tang, H. Xia, M. Cao, X. Zhang, W. Zeng, S. Hu, W. Tong, J. Wang, J. Wang, J. Yu, et al.
A Comparison of Rice Chloroplast Genomes
Plant Physiology, May 1, 2004; 135(1): 412 - 420.
[Abstract] [Full Text] [PDF]


Home page
Genome Res.Home page
T. Domazet-Loso and D. Tautz
An Evolutionary Analysis of Orphan Genes in Drosophila
Genome Res., October 1, 2003; 13(10): 2213 - 2219.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
C. Baar, M. Eppinger, G. Raddatz, J. Simon, C. Lanz, O. Klimmek, R. Nandakumar, R. Gross, A. Rosinus, H. Keller, et al.
Complete genome sequence and analysis of Wolinella succinogenes
PNAS, September 30, 2003; 100(20): 11690 - 11695.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
P. Cliften, P. Sudarsanam, A. Desikan, L. Fulton, B. Fulton, J. Majors, R. Waterston, B. A. Cohen, and M. Johnston
Finding Functional Features in Saccharomyces Genomes by Phylogenetic Footprinting
Science, July 4, 2003; 301(5629): 71 - 76.
[Abstract] [Full Text] [PDF]


Home page
Genome Res.Home page
N. Yuhki, T. Beck, R. M. Stephens, Y. Nishigaki, K. Newmann, and S. J. O'Brien
Comparative Genome Organization of Human, Murine, and Feline MHC Class II Region
Genome Res., June 1, 2003; 13(6): 1169 - 1179.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
B. J. Williams, M. Golomb, T. Phillips, J. Brownlee, M. V. Olson, and A. L. Smith
Bacteriophage HP2 of Haemophilus influenzae
J. Bacteriol., December 15, 2002; 184(24): 6893 - 6905.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
Y. Sasaki, J. Ishikawa, A. Yamashita, K. Oshima, T. Kenri, K. Furuya, C. Yoshino, A. Horino, T. Shiba, T. Sasaki, et al.
The complete genomic sequence of Mycoplasma penetrans, an intracellular bacterial pathogen in humans
Nucleic Acids Res., December 1, 2002; 30(23): 5293 - 5300.
[Abstract] [Full Text] [PDF]


Home page
ScienceHome page
E. Wienholds, S. Schulte-Merker, B. Walderich, and R. H. A. Plasterk
Target-Selected Inactivation of the Zebrafish rag1 Gene
Science, July 5, 2002; 297(5578): 99 - 102.
[Abstract] [Full Text] [PDF]


Home page
J. Bacteriol.Home page
C. K. Raymond, E. H. Sims, A. Kas, D. H. Spencer, T. V. Kutyavin, R. G. Ivey, Y. Zhou, R. Kaul, J. B. Clendenning, and M. V. Olson
Genetic Variation at the O-Antigen Biosynthetic Locus in Pseudomonas aeruginosa
J. Bacteriol., July 1, 2002; 184(13): 3614 - 3622.
[Abstract] [Full Text] [PDF]


Home page
Nucleic Acids ResHome page
Y. S. N. Butterfield, M. A. Marra, J. K. Asano, S. Y. Chan, R. Guin, M. I. Krzywinski, S. S. Lee, K. W. K. MacDonald, C. A. Mathewson, T. E. Olson, et al.
An efficient strategy for large-scale high-throughput transposon-mediated sequencing of cDNA clones
Nucleic Acids Res., June 1, 2002; 30(11): 2460 - 2468.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
A. I. Slesarev, K. V. Mezhevaya, K. S. Makarova, N. N. Polushin, O. V. Shcherbinina, V. V. Shakhova, G. I. Belova, L. Aravind, D. A. Natale, I. B. Rogozin, et al.
The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens
PNAS, April 2, 2002; 99(7): 4644 - 4649.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
J. C. Smoot, K. D. Barbian, J. J. Van Gompel, L. M. Smoot, M. S. Chaussee, G. L. Sylva, D. E. Sturdevant, S. M. Ricklefs, S. F. Porcella, L. D. Parkins, et al.
Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks
PNAS, March 21, 2002; (2002) 62526099.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page