Vol. 11, Issue 12, 2034-2040, December 2001
LETTER
Identification of Genes Encoding Amino Acid Permeases by Inactivation of Selected ORFs from the Synechocystis Genomic Sequence
María José
Quintero,
María Luz
Montesinos,
Antonia
Herrero, and
Enrique
Flores1
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo
Superior de Investigaciones Científicas-Universidad de Sevilla,
E-41092 Seville, Spain
Genes encoding elements of four amino acid permeases were identified
by insertional inactivation of ORFs from the genomic sequence of the
cyanobacterium Synechocystis sp. strain PCC 6803 whose
putative products are homologous to amino acid permease proteins from
other bacteria. A transport system for neutral amino acids and
histidine and a transport system for basic amino acids and glutamine
were identified as ABC-type transporters, whereas Na+-dependent transport of glutamate was found to be mediated
by at least two systems, the secondary permease GltS and a TRAP-type transporter. Except for GltS, substrate specificities of the identified permeases do not match those of previously characterized systems homologous to these permeases.
1
Corresponding author.
11:2034-2040 ©2001 by Cold Spring Harbor Laboratory Press ISSN 1088-9051/01 $5.00