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Published online before print October 15, 2001, 10.1101/gr.197301
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Vol. 11, Issue 11, 1817-1825, November 2001

LETTER
Sequence, Regulation, and Evolution of the Maize 22-kD alpha  Zein Gene Family

Rentao Song, Victor Llaca, Eric Linton, and Joachim Messing1

Waksman Institute, Rutgers, State University of New Jersey, Piscataway, New Jersey 08854, USA

We have isolated and sequenced all 23 members of the 22-kD alpha  zein (z1C) gene family of maize. This is one of the largest plant gene families that has been sequenced from a single genetic background and includes the largest contiguous genomic DNA from maize with 346,292 bp to date. Twenty-two of the z1C members are found in a roughly tandem array on chromosome 4S forming a dense gene cluster 168,489-bp long. The twenty-third copy of the gene family is also located on chromosome 4S at a site ~20 cM closer to the centromere and appears to be the wild-type allele of the floury-2 (fl2) mutation. On the basis of an analysis of maize cDNA databases, only seven of these genes appear to be expressed including the fl2 allele. The expressed genes in the cluster are interspersed with nonexpressed genes. Interestingly, some of the expressed genes differ in their transcriptional regulation. Gene amplification appears to be in blocks of genes explaining the rapid and compact expansion of the cluster during the evolution of maize.

[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AF090447, AF031569, and AF090446]


1 Corresponding author.


11:1817-1825 ©2001 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/01 $5.00

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