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Vol. 11, Issue 1, 3-11, January 2001

REVIEW
Pyrosequencing Sheds Light on DNA Sequencing

Mostafa Ronaghi

Genome Technology Center, Stanford University, Palo Alto, California 94304, USA

DNA sequencing is one of the most important platforms for the study of biological systems today. Sequence determination is most commonly performed using dideoxy chain termination technology. Recently, pyrosequencing has emerged as a new sequencing methodology. This technique is a widely applicable, alternative technology for the detailed characterization of nucleic acids. Pyrosequencing has the potential advantages of accuracy, flexibility, parallel processing, and can be easily automated. Furthermore, the technique dispenses with the need for labeled primers, labeled nucleotides, and gel-electrophoresis. This article considers key features regarding different aspects of pyrosequencing technology, including the general principles, enzyme properties, sequencing modes, instrumentation, and potential applications.

11:3-11 ©2001 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/01 $5.00
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