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Vol. 10, Issue 6, 776-788, June 2000

LETTER
An EST-enriched Comparative Map of Brassica oleracea and Arabidopsis thaliana

Tien-Hung Lan,1 Terrye A. DelMonte,1 Kim P. Reischmann,1 Joel Hyman,1 Stanley P. Kowalski,1,2 Jim McFerson,3,4 Stephen Kresovich,3,5 and Andrew H. Paterson1,6,7

1 Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843 USA; 3 Plant Genetic Resources Unit, U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS), Geneva, New York 14456 USA

A detailed comparative map of Brassica oleracea and Arabidopsis thaliana has been established based largely on mapping of Arabidopsis ESTs in two Arabidopsis and four Brassica populations. Based on conservative criteria for inferring synteny, "one to one correspondence" between Brassica and Arabidopsis chromosomes accounted for 57% of comparative loci. Based on 186 corresponding loci detected in B. oleracea and A. thaliana, at least 19 chromosome structural rearrangements differentiate B. oleracea and A. thaliana orthologs. Chromosomal duplication in the B. oleracea genome was strongly suggested by parallel arrangements of duplicated loci on different chromosomes, which accounted for 41% of loci mapped in Brassica. Based on 367 loci mapped, at least 22 chromosomal rearrangements differentiate B. oleracea homologs from one another. Triplication of some Brassica chromatin and duplication of some Arabidopsis chromatin were suggested by data that could not be accounted for by the one-to-one and duplication models, respectively. Twenty-seven probes detected three or more loci in Brassica, which represent 25.3% of the 367 loci mapped in Brassica. Thirty-one probes detected two or more loci in Arabidopsis, which represent 23.7% of the 262 loci mapped in Arabidopsis. Application of an EST-based, cross-species genomic framework to isolation of alleles conferring phenotypes unique to Brassica, as well as the challenges and opportunities in extrapolating genetic information from Arabidopsis to Brassica and to more distantly related crops, are discussed.


Present addresses: 2USDA-ARS, Beltsville, Maryland 20704 USA; 4Washington Fruit Tree Research Commission, Wenatchee, Washington 98801 USA; 5Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York 14850 USA; 6Applied Genetic Technology Center, Department of Crop and Soil Sciences, Department of Botany, and Department of Genetics, University of Georgia, Athens, Georgia 30602 USA.

7 Corresponding author.


10:776-788 ©2000 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/00 $5.00

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