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Vol. 10, Issue 5, 691-702, May 2000

LETTER
A Radiation Hybrid Map of the Cat Genome: Implications for Comparative Mapping

William J. Murphy,1,4 Shan Sun,1,2 Zhang-qun Chen,3 Naoya Yuhki,1 Deborah Hirschmann,1 Marilyn Menotti-Raymond,1 and Stephen J. O'Brien1

1 Laboratory of Genomic Diversity, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201 USA; 2 Graduate Program in Biology, George Mason University, Fairfax, Virginia 22030 USA; 3 Intramural Research Support Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201 USA

Ordered gene maps of mammalian species are becoming increasingly valued in assigning gene variants to function in human and animal models, as well as recapitulating the natural history of genome organization. To extend this power to the domestic cat, a radiation hybrid (RH) map of the cat was constructed integrating 424 Type I-coding genes with 176 microsatellite markers, providing coverage over all 20 feline chromosomes. Alignment of parallel RH maps of human and cat reveal 100 conserved segments ordered (CSOs) between the species, nearly three times the number observed with reciprocal chromosome painting analyses. The observed number is equivalent to theoretical predictions of the number of conserved segments to be found between cat and human, implying that 300-400 Type I gene markers is sufficient to reveal nearly all conserved segments for species that exhibit the most frequently observed "slow" rate of genome reorganization. The cat-human RH map comparisons provide a new genomic tool for comparative gene mapping in the cat and related Felidae, and provide confirmation that the cat genome organization is remarkably conserved compared with human. These data demonstrate that ordered RH-based gene maps provide the most precise assessment of comparing genomes, short of contig construction or full-sequence determination.

[The sequence data described in this paper have been submitted to the GenBank data library under accession nos. AZ081249-AZ081287 and AW646734-AW646956.]


4 Corresponding author.


10:691-702 ©2000 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/00 $5.00

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