Genome Research cityscape

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ishihara, K.
Right arrow Articles by Sasaki, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishihara, K.
Right arrow Articles by Sasaki, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 10, Issue 5, 664-671, May 2000

LETTER
Comparative Genomic Sequencing Identifies Novel Tissue-Specific Enhancers and Sequence Elements for Methylation-Sensitive Factors Implicated in Igf2/H19 Imprinting

Ko Ishihara,1,2,3 Naoya Hatano,3,7 Hiroyasu Furuumi,1,3 Reiko Kato,1,3,8 Toru Iwaki,4 Kiyonori Miura,5,9 Yoshihiro Jinno,5,6 and Hiroyuki Sasaki1,2,3,10

1 Division of Human Genetics, Department of Integrated Genetics, National Institute of Genetics and 2 Department of Genetics, Graduate University for Advanced Studies, Mishima, Shizuoka 411-8540, Japan; 3 Division of Disease Genes, Institute of Genetic Information, and 4 Department of Neuropathology, Neurological Institute, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan; 5 Department of Human Genetics, Nagasaki University School of Medicine, Nagasaki 852-8523, Japan; 6 Department of Molecular Biology, Ryukyu University School of Medicine, Nishihara, Okinawa 903-0215, Japan

A differentially methylated region (DMR) and endoderm-specific enhancers, located upstream and downstream of the mouse H19 gene, respectively, are known to be essential for the reciprocal imprinting of Igf2 and H19. To explain the same imprinting patterns in non-endodermal tissues, additional enhancers have been hypothesized. We determined and compared the sequences of human and mouse H19 over 40 kb and identified 10 evolutionarily conserved downstream segments, 2 of which were coincident with the known enhancers. Reporter assays in transgenic mice showed that 5 of the other 8 segments functioned as enhancers in specific mesodermal and/or ectodermal tissues. We also identified a conserved 39-bp element that appeared repeatedly within the DMR and formed complexes with specific nuclear factors. Binding of one of the factors was inhibited when the target sequence contained methylated CpGs. These complexes may contribute to the presumed boundary function of the unmethylated DMR, which is proposed to insulate maternal Igf2 from the enhancers. Our results demonstrate that comparative genomic sequencing is highly efficient in identifying regulatory elements.

[The sequence data described in this paper have been submitted to GenBank under accession nos. AF087017 and AF049091.]

Present addresses: 7National Institute of Bioscience and Human Technology, Ministry of International Trade and Industry, Tsukuba, Ibaraki 305-8566, Japan; 8 Human Genome Research Group, RIKEN Genomic Sciences Center, c/o Kitasato University, Sagamihara, Kanagawa 228-8555, Japan; 9 Department of Pediatrics, Harvard University Faculty of Medicine & Genetic Division, Department of Medicine, Children's Hospital, Boston, Massachusetts 02115 USA.

10 Corresponding author.

10:664-671 ©2000 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/00 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
B. Yoon, H. Herman, B. Hu, Y. J. Park, A. Lindroth, A. Bell, A. G. West, Y. Chang, A. Stablewski, J. C. Piel, et al.
Rasgrf1 Imprinting Is Regulated by a CTCF-Dependent Methylation-Sensitive Enhancer Blocker
Mol. Cell. Biol., December 15, 2005; 25(24): 11184 - 11190.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
F. M. Pauler, S. H. Stricker, K. E. Warczok, and D. P. Barlow
Long-range DNase I hypersensitivity mapping reveals the imprinted Igf2r and Air promoters share cis-regulatory elements
Genome Res., October 1, 2005; 15(10): 1379 - 1387.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
T. Yokomine, H. Shirohzu, W. Purbowasito, A. Toyoda, H. Iwama, K. Ikeo, T. Hori, S. Mizuno, M. Tsudzuki, Y.-i. Matsuda, et al.
Structural and functional analysis of a 0.5-Mb chicken region orthologous to the imprinted mammalian Ascl2/Mash2-Igf2-H19 region
Genome Res., January 1, 2005; 15(1): 154 - 165.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
M. Paulsen, T. Khare, C. Burgard, S. Tierling, and J. Walter
Evolution of the Beckwith-Wiedemann syndrome region in vertebrates
Genome Res., January 1, 2005; 15(1): 146 - 153.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Long and B. T. Spear
FoxA Proteins Regulate H19 Endoderm Enhancer E1 and Exhibit Developmental Changes in Enhancer Binding In Vivo
Mol. Cell. Biol., November 1, 2004; 24(21): 9601 - 9609.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. M. Wilson, M. M. Hsieh, and P. Rotwein
Autocrine Growth Factor Signaling by Insulin-like Growth Factor-II Mediates MyoD-stimulated Myocyte Maturation
J. Biol. Chem., October 17, 2003; 278(42): 41109 - 41113.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
T. C. Kaufman, D. W. Severson, and G. E. Robinson
The Anopheles Genome and Comparative Insect Genomics
Science, October 4, 2002; 298(5591): 97 - 98.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
K. Ishihara and H. Sasaki
An evolutionarily conserved putative insulator element near the 3' boundary of the imprinted Igf2/H19 domain
Hum. Mol. Genet., July 1, 2002; 11(14): 1627 - 1636.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
J. Cavaille, H. Seitz, M. Paulsen, A. C. Ferguson-Smith, and J.-P. Bachellerie
Identification of tandemly-repeated C/D snoRNA genes at the imprinted human 14q32 domain reminiscent of those at the Prader-Willi/Angelman syndrome region
Hum. Mol. Genet., June 15, 2002; 11(13): 1527 - 1538.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. L. Thorvaldsen, M. R. W. Mann, O. Nwoko, K. L. Duran, and M. S. Bartolomei
Analysis of Sequence Upstream of the Endogenous H19 Gene Reveals Elements Both Essential and Dispensable for Imprinting
Mol. Cell. Biol., April 15, 2002; 22(8): 2450 - 2462.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
K. Davies, L. Bowden, P. Smith, W. Dean, D. Hill, H. Furuumi, H. Sasaki, B. Cattanach, and W. Reik
Disruption of mesodermal enhancers for Igf2 in the minute mutant
Development, January 4, 2002; 129(7): 1657 - 1668.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
R. A. Drewell, K. L. Arney, T. Arima, S. C. Barton, J. D. Brenton, and M. A. Surani
Novel conserved elements upstream of the H19 gene are transcribed and act as mesodermal enhancers
Development, January 3, 2002; 129(5): 1205 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. R. Kaffer, A. Grinberg, and K. Pfeifer
Regulatory Mechanisms at the Mouse Igf2/H19 Locus
Mol. Cell. Biol., December 1, 2001; 21(23): 8189 - 8196.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
B. K. Jones, J. Levorse, and S. M. Tilghman
Deletion of a nuclease-sensitive region between the Igf2 and H19 genes leads to Igf2 misregulation and increased adiposity
Hum. Mol. Genet., April 1, 2001; 10(8): 807 - 814.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
J. W. Touchman, A. Dehejia, O. Chiba-Falek, D. E. Cabin, J. R. Schwartz, B. M. Orrison, M. H. Polymeropoulos, and R. L. Nussbaum
Human and Mouse alpha -Synuclein Genes: Comparative Genomic Sequence Analysis and Identification of a Novel Gene Regulatory Element
Genome Res., January 1, 2001; 11(1): 78 - 86.
[Abstract] [Full Text]

Home page
 Hum Mol GenetHome page
T. L. Davis, G. J. Yang, J. R. McCarrey, and M. S. Bartolomei
The H19 methylation imprint is erased and re-established differentially on the parental alleles during male germ cell development
Hum. Mol. Genet., November 1, 2000; 9(19): 2885 - 2894.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genes Dev. Learn. Mem.
Protein Science RNA Genome Res.