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Vol. 10, Issue 3, 344-349, March 2000

METHODS
The Human Adult Skeletal Muscle Transcriptional Profile Reconstructed by a Novel Computational Approach

Stefania Bortoluzzi,1 Fabio d'Alessi,1 Chiara Romualdi,2 and Gian Antonio Danieli1,3

1 Department of Biology and 2 Department of Statistics, University of Padua, 35131 Padua, Italy

By applying a novel software tool, information on 4080 UniGene clusters was retrieved from three adult human skeletal muscle cDNA libraries, which were selected for being neither normalized nor subtracted. Reconstruction of a transcriptional profile of the corresponding tissue was attempted by a computational approach, classifying each transcript according to its level of expression. About 25% of the transcripts accounted for about 80% of the detected transcriptional activity, whereas most genes showed a low level of expression. This in silico transcriptional profile was then compared with data obtained by a SAGE study. A fairly good agreement between the two methods was observed. About 400 genes, highly expressed in skeletal muscle or putatively skeletal muscle-specific, may represent the minimal set of genes needed to determine the tissue specificity. These genes could be used as a convenient reference to monitor major changes in the transcriptional profile of adult human skeletal muscle in response to different physiological or pathological conditions, thus providing a framework for designing DNA microarrays and initiating biological studies.

3 Corresponding author.

10:344-349 ©2000 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/00 $5.00
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