Genome Research

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

Published online before print November 8, 2000, 10.1101/gr.GR-1405R
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
GR-1405Rv1
10/11/1743    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thomson, T. M.
Right arrow Articles by Guigó, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thomson, T. M.
Right arrow Articles by Guigó, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Vol. 10, Issue 11, 1743-1756, November 2000

LETTER
Fusion of the Human Gene for the Polyubiquitination Coeffector UEV1 with Kua, a Newly Identified Gene

Timothy M. Thomson,1,2,7,8 Juan José Lozano,1,2,3,7 Noureddine Loukili,1,2,7 Roberto Carrió,4 Florenci Serras,5 Bru Cormand,2,6 Marta Valeri,2 Víctor M. Díaz,1,2 Josep Abril,3 Moisés Burset,3 Jesús Merino,4 Alfons Macaya,2,6 Montserrat Corominas,5 and Roderic Guigó3

1 Institut de Biologia Molecular, Consejo Superior de Investigaciones Cientificas, Barcelona, Spain; 2 Unitat de Recerca Biomèdica, Hospital Materno-Infantil, Hospitals Vall d'Hebrón, Barcelona, Spain; 3 Grup de Recerca en Informática Mèdica, Institut Municipal d'Investigació Mèdica, Universitat Pompeu Fabra, Barcelona, Spain; 4 Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain; 5 Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain; 6 Unitat de Malaties Neurometabòliques, Hospital Materno-Infantil, Hospitals Vall d'Hebrón, Barcelona, Spain

UEV proteins are enzymatically inactive variants of the E2 ubiquitin-conjugating enzymes that regulate noncanonical elongation of ubiquitin chains. In Saccharomyces cerevisiae, UEV is part of the RAD6-mediated error-free DNA repair pathway. In mammalian cells, UEV proteins can modulate c-FOS transcription and the G2-M transition of the cell cycle. Here we show that the UEV genes from phylogenetically distant organisms present a remarkable conservation in their exon-intron structure. We also show that the human UEV1 gene is fused with the previously unknown gene Kua. In Caenorhabditis elegans and Drosophila melanogaster, Kua and UEV are in separated loci, and are expressed as independent transcripts and proteins. In humans, Kua and UEV1 are adjacent genes, expressed either as separate transcripts encoding independent Kua and UEV1 proteins, or as a hybrid Kua-UEV transcript, encoding a two-domain protein. Kua proteins represent a novel class of conserved proteins with juxtamembrane histidine-rich motifs. Experiments with epitope-tagged proteins show that UEV1A is a nuclear protein, whereas both Kua and Kua-UEV localize to cytoplasmic structures, indicating that the Kua domain determines the cytoplasmic localization of Kua-UEV. Therefore, the addition of a Kua domain to UEV in the fused Kua-UEV protein confers new biological properties to this regulator of variant polyubiquitination.

[Kua cDNAs isolated by RT-PCR and described in this paper have been deposited in the GenBank data library under accession nos. AF1155120 (H. sapiens) and AF152361 (D. melanogaster). Genomic clones containing UEV genes: S. cerevisiae, YGL087c (accession no. Z72609); S. pombe, c338 (accession no. AL023781); P. falciparum, MAL3P2 (accession no. AL034558); A. thaliana, F26F24 (accession no. AC005292); C. elegans, F39B2 (accession no. Z92834); D. melanogaster, AC014908; and H. sapiens, 1185N5 (accession no. AL034423). Accession numbers for Kua cDNAs in GenBank dbEST: M. musculus, AA7853; T. cruzi, AI612534. Other Kua-containing sequences: A. thaliana genomic clones F10M23 (accession no. AL035440), F19K23 (accession no. AC000375), and T20K9 (accession no. AC004786).]

7 These authors have contributed equally to this work.

8 Corresponding author.

10:1743-1756 ©2000 by Cold Spring Harbor Laboratory Press  ISSN 1088-9051/00 $5.00
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
L. Galbis-Martinez, M. Galbis-Martinez, F. J. Murillo, and M. Fontes
An anti-antisigma factor in the response of the bacterium Myxococcus xanthus to blue light
Microbiology, March 1, 2008; 154(3): 895 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Hernandez-Sanchez, O. Bartulos, A. I. Valenciano, A. Mansilla, and F. de Pablo
The regulated expression of chimeric tyrosine hydroxylase-insulin transcripts during early development
Nucleic Acids Res., July 13, 2006; 34(12): 3455 - 3464.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
V. Katju and M. Lynch
On the Formation of Novel Genes by Duplication in the Caenorhabditis elegans Genome
Mol. Biol. Evol., May 1, 2006; 23(5): 1056 - 1067.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
P. Akiva, A. Toporik, S. Edelheit, Y. Peretz, A. Diber, R. Shemesh, A. Novik, and R. Sorek
Transcription-mediated gene fusion in the human genome
Genome Res., January 1, 2006; 16(1): 30 - 36.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
G. Parra, A. Reymond, N. Dabbouseh, E. T. Dermitzakis, R. Castelo, T. M. Thomson, S. E. Antonarakis, and R. Guigo
Tandem chimerism as a means to increase protein complexity in the human genome
Genome Res., January 1, 2006; 16(1): 37 - 44.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. D. Jones, A. W. Custer, and D. J. Begun
Origin and Evolution of a Chimeric Fusion Gene in Drosophila subobscura, D. madeirensis and D. guanche
Genetics, May 1, 2005; 170(1): 207 - 219.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Salaun, S. Boulben, O. Mulner-Lorillon, R. Belle, N. Sonenberg, J. Morales, and P. Cormier
Embryonic-stage-dependent changes in the level of eIF4E-binding proteins during early development of sea urchin embryos
J. Cell Sci., April 1, 2005; 118(7): 1385 - 1394.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
F. D. Ciccarelli, C. von Mering, M. Suyama, E. D. Harrington, E. Izaurralde, and P. Bork
Complex genomic rearrangements lead to novel primate gene function
Genome Res., March 1, 2005; 15(3): 343 - 351.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Teo, D. B. Veprintsev, and R. L. Williams
Structural Insights into Endosomal Sorting Complex Required for Transport (ESCRT-I) Recognition of Ubiquitinated Proteins
J. Biol. Chem., July 2, 2004; 279(27): 28689 - 28696.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Poulin, A. Brueschke, and N. Sonenberg
Gene Fusion and Overlapping Reading Frames in the Mammalian Genes for 4E-BP3 and MASK
J. Biol. Chem., December 26, 2003; 278(52): 52290 - 52297.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
V. Katju and M. Lynch
The Structure and Early Evolution of Recently Arisen Gene Duplicates in the Caenorhabditis elegans Genome
Genetics, December 1, 2003; 165(4): 1793 - 1803.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
Y. Zhuang, F. Ma, J. Li-Ling, X. Xu, and Y. Li
Comparative Analysis of Amino Acid Usage and Protein Length Distribution Between Alternatively and Non-alternatively Spliced Genes Across Six Eukaryotic Genomes
Mol. Biol. Evol., December 1, 2003; 20(12): 1978 - 1985.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
A. Courseaux and J.-L. Nahon
Birth of Two Chimeric Genes in the Hominidae Lineage
Science, February 16, 2001; 291(5507): 1293 - 1297.
[Abstract] [Full Text]

Home page
 Genome Res.Home page
M. Long
A New Function Evolved from Gene Fusion
Genome Res., November 1, 2000; 10(11): 1655 - 1657.
[Full Text]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genes Dev. Learn. Mem.
Protein Science RNA Genome Res.