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PCR Methods Appl. 1:43-45, 1991
©1991 by Cold Spring Harbor Laboratory Press; ISSN 1088-9051
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A PCR-enhanced method for determining the 5' end sequence of mRNAs.

M A Hofmann and D A Brian

Department of Microbiology, University of Tennessee, Knoxville 37996-0845.

Abstract

A method was developed to amplify ligated cDNA copies of mRNA 5' ends before cloning and sequencing. The method facilitates the study of the 5' end sequence on many molecules of an individual mRNA species. It can also be used to study the 5' end sequence on other kinds of single-stranded RNA molecules.



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